AB181046

重组Anti-Importin4/Imp4抗体[EPR13660]

Anti-Importin4/Imp4 antibody [EPR13660]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal Importin4/Imp4 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

查看别名

IMP4B, RANBP4, IPO4, Importin-4, Imp4, Importin-4b, Ran-binding protein 4, Imp4b, RanBP4

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling Importin4/Imp4 using ab181046 at a 1/10 dilution (red) or a rabbit IgG control (green).

Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Immunofluorescent staining of A549 cells labeling Importin4/Imp4 using ab181046 at a 1/50 dilution (red) and DAPI nuclear staining (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labelling Importin4/Imp4 immunoprecipitated using ab181046 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Predicted band size: 119 kDa

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Unknown

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

Lanes 1-4 : Merged signal (red and green). Green - ab181046 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181046 Anti-Importin4/Imp4 antibody [EPR13660] was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266175 (knockout cell lysate ab258005) was used. Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE. ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

IPO4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell line (<a href='/products/cell-lines/human-ipo4-importin4-imp4-knockout-hek-293t-cell-line-ab266175'>ab266175</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 119 kDa

Observed band size: 130 kDa

false

Supplier Data

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

All lanes:

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/100000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

Human seminoma lysate at 10 µg

Predicted band size: 119 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Importin4/Imp4 antibody [EPR13660] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13660

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra), IP, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Importin4 (Imp4) also known as Ipo4 is a nuclear import receptor with an approximate mass of 130 kDa. This protein facilitates the transport of molecules such as ribosomal proteins and histones into the nucleus. Expression of Importin4 occurs in various tissues including the brain testis and liver. Importin4 plays a significant mechanical role by recognizing nuclear localization signals on cargo molecules and mediating their translocation across the nuclear envelope.

Biological function summary

Importin4 functions as part of the nuclear transport system a complex network essential for cellular operations. Importin4 interacts with the nuclear pore complex to enable import of specific protein cargo into the nucleus. By partnering with karyopherins and Ran-GTPase Importin4 participates in the active transport mechanism ensuring the correct localization of proteins that are critical to genomic regulation processes.

Pathways

Importin4 plays a role in the nuclear transport pathway and is associated with the ribosome biogenesis pathway. This protein works alongside proteins like Ran-GTPase enhancing the translocation of ribosomal components pivotal for cellular growth and protein synthesis. Importin4 also aids in the shuttling of histones which are essential to chromatin assembly and DNA transcription regulation.

Importin4 has connections to cancer and neurodegenerative diseases. Altered expression and mutations in Importin4 may disrupt cellular homeostasis and nuclear transport linking it to tumorigenesis. Additionally associations with proteins such as karyopherin alpha importers indicate its involvement in pathological states affecting the nervous system potentially contributing to conditions such as Alzheimer's disease. Understanding Importin4's interactions could provide insights into these complex diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR (PubMed : 11823430, PubMed : 16207705, PubMed : 17682055, PubMed : 21454524). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 11823430, PubMed : 16207705). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 11823430, PubMed : 16207705). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed : 11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 11823430). Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B) (PubMed : 21454524, PubMed : 29408485). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR) (PubMed : 16207705). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS (PubMed : 12610148).

See full target information IPO4

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 46:3839-3865 PubMed38785507

2024

Prognostic Value of Histone Acetyl Transferase 1 (HAT-1) and Inflammatory Signatures in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Laura Jiménez-Álvarez,Oscar Fraile-Martinez,Cielo Garcia-Montero,Luis G Guijarro,Leonel Pekarek,Silvestra Barrena-Blázquez,Ángel Asúnsolo,Laura López-González,María Del Val Toledo-Lobo,Melchor Álvarez-Mon,Miguel A Saez,Alberto Gutiérrez-Calvo,Raúl Díaz-Pedrero

Journal of oncology 2022:8545441 PubMed36317123

2022

IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruzhen Li,Zhaohui Han,Wei Ma,Lin Zhang,Xiangwei Zhang,Yuanzhu Jiang,Wei Dong

Journal of cell science 134: PubMed33912945

2021

On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Kalita,Larisa E Kapinos,Roderick Y H Lim

Atherosclerosis 284:223-229 PubMed30777337

2019

A new variant (c.1A>G) in LDLRAP1 causing autosomal recessive hypercholesterolemia: Characterization of the defect and response to PCSK9 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Rodríguez-Jiménez,Diego Gómez-Coronado,Manuel Frías Vargas,Francisca Cerrato,Carlos Lahoz,Jose Saban-Ruiz,Daniel González-Nieto,Miguel A Lasunción,José M Mostaza,Sonia Rodríguez-Nóvoa

View all publications

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重组Anti-Importin4/Imp4抗体[EPR13660]