重组Anti-Importin 9/RANBP9抗体[EPR1352] (ab124710)
![Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.cn/ps/products/124/ab124710/Images/ab124710-392195-anti-ipo9-epr1352-antibody-western-blot-wt-hela-ipo9-ko-hela-sk-br-3-cells.jpg "Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Importin 9/RANBP9抗体[EPR1352]
参阅全部 Importin 9/RANBP9 一抗 -
描述
兔单克隆抗体[EPR1352] to Importin 9/RANBP9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.29 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1352 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124710于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6. -
序列相似性
Belongs to the importin beta family.
Contains 1 importin N-terminal domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55705 Human
- Entrez Gene: 226432 Mouse
- Entrez Gene: 304817 Rat
- SwissProt: Q96P70 Human
- SwissProt: Q91YE6 Mouse
- Unigene: 596014 Human
- Unigene: 271642 Mouse
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别名
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
see all
图片
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Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IPO9 knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 116 kDaLanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.
ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.cn/ps/products/124/ab124710/Images/ab124710-138511-anti-importin-9-antibody-epr1352-western-blot.jpg)
Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.cn/ps/products/124/ab124710/Images/ab124710-138510-anti-importin-9-antibody-epr1352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.cn/ps/products/124/ab124710/Images/ab124710-138509-anti-importin-9-antibody-epr1352.jpg)
OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) -
![Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.cn/ps/products/124/ab124710/Images/ab124710-5-benefits-of-recombinant-antibodies.png)
Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124710 被引用在 2 文献中.
- Hirai Y et al. Intracellular dynamics of actin affects Borna disease virus replication in the nucleus. Virus Res 263:179-183 (2019). PubMed: 30769121
- Liu C et al. Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading. iScience 9:314-327 (2018). PubMed: 30448731