AB325441

Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal IMPDH1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Transfected cell lysate - Human samples.

IMPD1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling IMPDH1 with ab325431 at 1/5000 (0.101 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human retina. (IS : inner segment, ONL : outer nuclear layer, OPL : outer plexiform layer, IPL : inner plexiform layer, INL : inner nuclear layer, GCL : ganglion cell layer)

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded A : HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human IMPDH1 expression vector containing a Myc-His tag B : HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human IMPDH2 expression vector containing a Myc-His tag C : HEK-293T transfected with empty vector containing a Myc-His tag tissue labeling IMPDH1 with ab325431 at 1/5000 (0.101 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in A : HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human IMPDH1 expression vector containing a Myc-His tag, and no signal in B : HEK-293T transfected with a human IMPDH2 expression vector containing a Myc-His tag and C : HEK-293T transfected with empty vector containing a Myc-His tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling IMPDH1 with ab325431 at 1/5000 (0.101 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse retina. (IS : inner segment, ONL : outer nuclear layer, OPL : outer plexiform layer, IPL : inner plexiform layer, INL : inner nuclear layer, GCL : ganglion cell layer)

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Lab

Western blot - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab325431 was shown to bind specifically to IMPDH1. Target of interest was observed at 55 kDa in wild-type HAP1 cell lysates (lane 3) with no signal observed at this size in IMPDH1 knockout cell line (lane 4).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes1-5 : 180 seconds, lane6 : 26 seconds

All lanes:

Western blot - Anti-IMPDH1 antibody [EPR30383-519] (<a href='/products/primary-antibodies/impdh1-antibody-epr30383-519-ab325431'>ab325431</a>) at 1/1000 dilution

Lane 1:

LN-229 (human brain glioblastoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Lane 2:

LN-229 transfected with siRNA specifically targeting IMPDH1 whole cell lysate at 80 µg

Lane 3:

parental HAP1(human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 80 µg

Lane 4:

IMPDH1 KO HAP1 whole cell lysate at 80 µg

Lane 5:

786-O (human kidney epithelial cell) whole cell lysate at 80 µg

Lane 6:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

false

Lab

Western blot - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human IMPDH2 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IMPDH1 antibody [EPR30383-519] (<a href='/products/primary-antibodies/impdh1-antibody-epr30383-519-ab325431'>ab325431</a>) at 1/1000 dilution

Lane 1:

His-tag purified protein overexpressed in 293T human embryonic kidney epithelial cells, transfected with a human IMPDH1 expression vector incorporating a Myc-His® tag, whole-cell lysate at 20 µg

Lane 2:

His-tag purified protein overexpressed in 293T human embryonic kidney epithelial cells, transfected with a human IMPDH2 expression vector incorporating a Myc-His® tag, whole-cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 48s

Lab

Western blot - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver(PMID : 32702086)

The identity of the higher MW bands at approximately 64 kDa corresponds to the 603/604aa retinal-specific spliced form(PMID : 32254022, 16936083)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes1-2 : 59 seconds, lane3 : 180 seconds, lane4 : 26seconds

All lanes:

Western blot - Anti-IMPDH1 antibody [EPR30383-519] (<a href='/products/primary-antibodies/impdh1-antibody-epr30383-519-ab325431'>ab325431</a>) at 1/1000 dilution

Lane 1:

Mouse pancreas tissue lysate at 80 µg

Lane 2:

Mouse liver tissue lysate at 80 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Lane 4:

Mouse retina tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,64 kDa,36 kDa

false

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling IMPDH1 with ab325431 at 1/5000 (0.101 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free (AB325441)

This data was developed using ab325431, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling IMPDH1 with ab325431 at 1/5000 (0.101 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR30383-519

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with overexpressed human IMPDH2 in Western blot.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

See full target information IMPDH1

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IMPDH1 antibody [EPR30383-519] - BSA and Azide free