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AB69311

Anti-IMPAD1抗体

Anti-IMPAD1 antibody

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(2 Publications)

Mouse Polyclonal IMPAD1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BPNT2.

查看别名

IMPA3, IMPAD1, BPNT2, Golgi-resident PAP phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Myo-inositol monophosphatase A3, Phosphoadenosine phosphate 3'-nucleotidase

1 Images
Western blot - Anti-IMPAD1 antibody (AB69311)
  • WB

Unknown

Western blot - Anti-IMPAD1 antibody (AB69311)

All lanes:

Western blot - Anti-IMPAD1 antibody (ab69311) at 1/500 dilution

Lane 1:

IMPAD1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human BPNT2.

Q9NX62

特异性

This antibody is reactive against mammalian transfected lysate.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IMPAD1 also known as Inositol Monophosphatase Domain Containing 1 is an enzyme with a molecular mass of approximately 50 kDa. The enzyme is primarily located in the Golgi apparatus of cells. It catalyzes the dephosphorylation of inositol monophosphates which is a critical step in the phosphatidylinositol signaling pathway. The expression of IMPAD1 occurs in various tissues including the brain cartilage and kidney reflecting its diverse roles in different biological functions.
Biological function summary

IMPAD1 contributes to maintaining cellular inositol balance and is involved in the regulation of signaling pathways. It does not directly participate in a complex but its activity is essential for sustaining the levels of inositol phosphates within the cell. This function is particularly significant in neural and cartilage cells where it helps in the modulation of cellular responses to external stimuli.

Pathways

IMPAD1 plays a role in the phosphoinositide signaling pathway and the inositol phosphate metabolism pathway. It works alongside other phosphatases and kinases including Inositol polyphosphate-4-phosphatase type II (INPP4B) to regulate the phosphorylation state of inositol phosphates. Through these pathways it influences the synthesis of important secondary messengers like PI(345)P3 which are important for cellular signaling processes.

IMPAD1 has been associated with chondrodysplasia with joint dislocations and neurodegenerative disorders. Mutations in the IMPAD1 gene can disrupt the inositol metabolism leading to severe skeletal abnormalities and joint issues. Additionally dysfunction in its related signaling pathways particularly through interactions with proteins like INPP4B can contribute to neurodegenerative pathologies highlighting its broad impact on human health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Exhibits 3'-nucleotidase activity toward adenosine 3',5'-bisphosphate (PAP), namely hydrolyzes adenosine 3',5'-bisphosphate into adenosine 5'-monophosphate (AMP) and a phosphate. May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Has no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3 and I(1,3,4,5)P4.
See full target information BPNT2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Molecular therapy. Nucleic acids 29:969-978 PubMed36189081

2022

Anti-TGF-β1 aptamer enhances therapeutic effect of tyrosine kinase inhibitor, gefitinib, on non-small cell lung cancer in xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Takahashi,Yoshifumi Hashimoto,Yoshikazu Nakamura
View all publications
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