重组Anti-IMP3抗体[EPR12022] (ab177942)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12022] to IMP3
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IMP3抗体[EPR12022]
参阅全部 IMP3 一抗 -
描述
兔单克隆抗体[EPR12022] to IMP3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Recombinant fragment within Human IMP3. The exact sequence is proprietary.
Database link: O00425 -
阳性对照
- HeLa, 293T, K562 and HT-29 cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12022 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa). |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
靶标
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功能
RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. -
组织特异性
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. -
序列相似性
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
结构域
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
细胞定位
Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. - Information by UniProt
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数据库链接
- Entrez Gene: 10643 Human
- Entrez Gene: 312320 Rat
- Omim: 608259 Human
- SwissProt: O00425 Human
- Unigene: 700696 Human
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别名
- Cancer/testis antigen 98 antibody
- CT98 antibody
- DKFZp686F1078 antibody
see all
图片
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All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/2000 dilution
Lane 1 : Wild-type HCT 116 cell lysate
Lane 2 : IGF2BP3 knockout HCT 116 cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : IGF2BP3 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 60-75 kDa why is the actual band size different from the predicted?Western blot: Anti-IGF2BP3 antibody [EPR12022] (ab177942) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab177942 was shown to bind specifically to IGF2BP3. A band was observed at 60-75 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in IGF2BP3 knockout cell line. To generate this image, wild-type and IGF2BP3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab177942 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab177942 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177942 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent analysis of HeLa cells labeling IMP3 with ab177942 at 1/50 dilution (green). DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab177942.
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All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab177942 被引用在 3 文献中.
- Xu P et al. LncRNA AGAP2 antisense RNA 1 stabilized by insulin-like growth factor 2 mRNA binding protein 3 promotes macrophage M2 polarization in clear cell renal cell carcinoma through regulation of the microRNA-9-5p/THBS2/PI3K-Akt pathway. Cancer Cell Int 23:330 (2023). PubMed: 38110984
- Li K et al. A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability. Nat Commun 14:6876 (2023). PubMed: 37898647
- Mizutani R et al. Oncofetal protein IGF2BP3 facilitates the activity of proto-oncogene protein eIF4E through the destabilization of EIF4E-BP2 mRNA. Oncogene 35:3495-502 (2016). PubMed: 26522719