Anti-IMP3抗体(ab273131)
Key features and details
- Rabbit polyclonal to IMP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IMP3抗体
参阅全部 IMP3 一抗 -
描述
兔多克隆抗体to IMP3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human IMP3 aa 100-250.
Database link: O00425 -
阳性对照
- IHC-P: Human placenta and testis tissue. WB: NTERA-2 cells. ICC/IF: U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273131于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 63 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 63 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. -
组织特异性
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. -
序列相似性
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
结构域
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
细胞定位
Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. - Information by UniProt
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数据库链接
- Entrez Gene: 10643 Human
- Omim: 608259 Human
- SwissProt: O00425 Human
- Unigene: 700696 Human
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别名
- Cancer/testis antigen 98 antibody
- CT98 antibody
- DKFZp686F1078 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody (ab273131)
PFA-fixed, Triton X-100-permeabilized U-251 MG (Human brain glioma cell line) cells stained for IMP3 using ab273131 at 2 µg/ml (green) in ICC/IF.
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Western blot - Anti-IMP3 antibody (ab273131)Anti-IMP3 antibody (ab273131) at 0.4 µg/ml + NTERA-2 (Human cell line) cell lysate
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody (ab273131)Paraffin-embedded human testis tissue stained for IMP3 using ab273131 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody (ab273131)Paraffin-embedded human placenta tissue stained for IMP3 using ab273131 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody (ab273131)Paraffin-embedded human skeletal muscle tissue stained for IMP3 using ab273131 at 1/50 dilution in immunohistochemical analysis.
Shows no positivity in myocytes as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody (ab273131)Paraffin-embedded human liver tissue stained for IMP3 using ab273131 at 1/50 dilution in immunohistochemical analysis.
Shows no positivity in hepatocytes as expected.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab273131 被引用在 1 文献中.
- Zhao H et al. LncRNA FOXD3-AS1/miR-128-3p axis-mediated IGF2BP3 in glioma stimulates cancer angiogenesis and progression. Folia Neuropathol 61:168-184 (2023). PubMed: 37587892