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重组RabMAb

重组Anti-ILT-3抗体[EPR22030] (ab224716)

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Flow Cytometry - Anti-ILT-3 antibody [EPR22030] (ab224716)
  • Anti-ILT-3 antibody [EPR22030] (ab224716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22030] to ILT-3
  • Suitable for: Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ILT-3抗体[EPR22030]
    参阅全部 ILT-3 一抗

  • 描述

    兔单克隆抗体[EPR22030] to ILT-3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cytmore details
    不适用于: ICC/IF,IHC-P or WB

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt: Human peripheral blood mononuclear cells (PBMCs).

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab224716于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/600.
说明
Flow Cyt
1/600.
应用说明
Is unsuitable for ICC/IF,IHC-P or WB.

靶标

  • 功能

    Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

  • 组织特异性

    Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.

  • 序列相似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 结构域

    Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • 细胞定位

    Cell membrane. Ligand binding leads to internalization and translocation to an antigen-processing compartment.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD85 antigen-like family member K antibody
      • CD85k antibody
      • HM18 antibody
      • ILT-3 antibody
      • ILT3 antibody
      • Immunoglobulin-like transcript 3 antibody
      • Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 antibody
      • Leukocyte immunoglobulin-like receptor 5 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 4 antibody
      • LILRB4 antibody
      • LILRB5 antibody
      • LIR-5 antibody
      • LIR5 antibody
      • LIRB4_HUMAN antibody
      • Monocyte inhibitory receptor HM18 antibody
      see all

    图片

    • Flow Cytometry - Anti-ILT-3 antibody [EPR22030] (ab224716)
      Flow Cytometry - Anti-ILT-3 antibody [EPR22030] (ab224716)

      Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) labeling ILT-3 with ab224716 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with Alexa Fluor® 647-conjugated CD14 (a monocyte marker) and rabbit IgG (left, ab172370) or anti-ILT-3 RabMAb (right, ab224716). Data shown was gated on viable cells.

      The ILT-3 expression pattern has been described in literature (PMID:17266150; PMID:9151699).

    • Anti-ILT-3 antibody [EPR22030] (ab224716)
      Anti-ILT-3 antibody [EPR22030] (ab224716)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab224716？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab224716 尚未被引用在任何文献中。

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