重组Anti-ILF3抗体[EPR3627] (ab133354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3627] to ILF3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ILF3抗体[EPR3627]
参阅全部 ILF3 一抗 -
描述
兔单克隆抗体[EPR3627] to ILF3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human ILF3 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q12906 -
阳性对照
- WB: K-562, Raji, HeLa and A431 whole cell lysates; IHC-P: Human colon cancer; ICC/IF: A431 cells; Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3627 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133354于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50.
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WB |
1/2000 - 1/5000. Predicted molecular weight: 95 kDa.
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IHC-P |
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
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ICC/IF |
1/50 - 1/250.
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说明 |
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Flow Cyt (Intra)
1/50. |
WB
1/2000 - 1/5000. Predicted molecular weight: 95 kDa. |
IHC-P
1/6000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
ICC/IF
1/50 - 1/250. |
靶标
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功能
May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
组织特异性
Ubiquitous. -
序列相似性
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
翻译后修饰
Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 3609 Human
- Omim: 603182 Human
- SwissProt: Q12906 Human
- Unigene: 465885 Human
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别名
- CBTF antibody
- Double stranded RNA binding protein 76 antibody
- Double-stranded RNA-binding protein 76 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3627] (ab133354)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling ILF3 with Purified ab133354 at 1/6000 dilution (0.09 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-ILF3 antibody [EPR3627] (ab133354) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?ab133354 recognizes only NF110 isoform of ILF3.
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Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (2.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-ILF3 antibody [EPR3627] (ab133354) at 1/20000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?ab133354 recognizes only NF110 isoform of ILF3.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133354 被引用在 4 文献中.
- Li P et al. Characterization of circSCL38A1 as a novel oncogene in bladder cancer via targeting ILF3/TGF-β2 signaling axis. Cell Death Dis 14:59 (2023). PubMed: 36697384
- Toki N et al. SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies. Nucleic Acids Res 48:11626-11644 (2020). PubMed: 33130894
- Liu Y et al. Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop. J Exp Clin Cancer Res 38:439 (2019). PubMed: 31747963
- Sokol ES et al. SMARCE1 is required for the invasive progression of in situ cancers. Proc Natl Acad Sci U S A 114:4153-4158 (2017). WB ; Human . PubMed: 28377514