重组Anti-IL36 gamma/IL-1F9抗体[EPR23333-107] (ab272731)
![Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.cn/ps/products/272/ab272731/Images/ab272731-392016-anti-il36-gammail1f9-antibody-epr23333107-western-blot-hacat.jpg "Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-IL36 gamma/IL-1F9抗体[EPR23333-107]
参阅全部 IL36 gamma/IL-1F9 一抗 -
描述
兔单克隆抗体[EPR23333-107] to IL36 gamma/IL-1F9 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HaCaT (treated with 20ng/ml IL-1beta and 50ng/ml TNFalpha for 20 hours,) whole cell lysate; His-tagged recombinant human IL36G protein (aa18-169).
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23333-107 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). |
靶标
-
功能
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
组织特异性
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
序列相似性
Belongs to the IL-1 family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 56300 Human
- Omim: 605542 Human
- SwissProt: Q9NZH8 Human
- Unigene: 211238 Human
-
别名
- IL 1 epsilon antibody
- IL 1 related protein 2 antibody
- IL 1(EPSILON) antibody
see all
图片
-
Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)All lanes : Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte), whole cell lysate
Lane 2 : HaCaT treated with 20ng/ml IL-1beta and 50ng/ml TNF-alpha for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of IL36 gamma/IL-1F9 is induced by IL-1beta and TNF-alpha treatment in HaCaT (PMID: 23095752).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
-
![Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.cn/ps/products/272/ab272731/Images/ab272731-392017-anti-il36-gammail1f9-antibody-epr23333107-western-blot-histagged-recombinant-human-il36g-protein.jpg)
Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution + His-tagged recombinant human IL36G protein (aa18-169), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
-
![Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.cn/ps/products/272/ab272731/Images/ab272731-3-benefits-of-recombinant-antibodies.png)
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab272731 尚未被引用在任何文献中。