AB273155

Anti-IL27RA/WSX-1 抗体 [EPR20863-3] - BSA and Azide free

Anti-IL27RA/WSX-1 antibody [EPR20863-3] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal WSX-1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

查看别名

Tccr, Wsx1, Il27ra, Interleukin-27 receptor subunit alpha, IL-27 receptor subunit alpha, IL-27R subunit alpha, IL-27R-alpha, IL-27RA, Type I T-cell cytokine receptor, WSX-1, TCCR

1 Images

Lab

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] - BSA and Azide free (AB273155)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Data from collaborator.

Exposure time : 1 minute.

WSX-1 = IL27Ra

These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (ab220359).

All lanes:

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (<a href='/products/primary-antibodies/il27ra-wsx-1-antibody-epr20863-3-ab220359'>ab220359</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse spleen (1) tissue lysate at 20 µg

Lane 2:

Wild-type mouse spleen (2) tissue lysate at 20 µg

Lane 3:

Wild-type mouse spleen (3) tissue lysate at 20 µg

Lane 4:

IL27Ra knockout mouse spleen (1) tissue lysate at 20 µg

Lane 5:

IL27Ra knockout mouse spleen (2) tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 110-120 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-IL27RA/WSX-1 antibody [EPR20863-3]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20863-3

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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产品详情

ab273155 is the carrier-free version of ab220359.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The WSX-1 protein also known as IL-27Rα or TCCR is a subunit of the interleukin-27 receptor. It weighs approximately 74 kDa and is mainly expressed on various immune cells including T cells B cells and monocytes. WSX-1 engages in the immune signaling by interacting specifically with its ligand IL-27 to drive immunological responses. This receptor belongs to the type I cytokine receptor family and is structurally characterized by certain conserved motifs important for its signaling functions.

Biological function summary

WSX-1 plays a role in regulating immune cell activity and differentiation. It acts as a part of a receptor complex with gp130 to form the IL-27 receptor which transmits signals that modulate the activity of T helper cells. This signaling influences the production of pro-inflammatory and anti-inflammatory cytokines impacting immune system balance. The function of WSX-1 remains critical for controlling inflammatory responses and ensuring immune tolerance.

Pathways

WSX-1 contributes to the JAK/STAT signaling pathway and influences the differentiation of Th1 cells. The connection to JAK/STAT signaling involves various proteins such as STAT1 and STAT3 which transduce IL-27-induced signals leading to gene expression changes in immune cells. Through its action in these pathways WSX-1 regulates immune responses and can influence inflammation dynamics in various biological contexts.

WSX-1 has associations with autoimmune diseases and infectious conditions. Alterations in its signaling pathways can lead to dysregulated immune responses contributing to diseases like multiple sclerosis and inflammatory bowel disease. The protein’s interaction with IL-27 is central in these conditions as it influences the pro-inflammatory and regulatory functions of the immune system. Furthermore studies suggest a link between WSX-1 activity and the modulation of responses against infections linking it to potential therapeutic targets for managing related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for IL27. Requires IL6ST/GP130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

See full target information Il27ra

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Blood 136:183-198 PubMed32305041

2020

Aging-induced IL27Ra signaling impairs hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqing He,Panglian Xu,Xiaofei Zhang,Min Liao,Qiongye Dong,Tingting Cong,Baixue Tang,Xiuxiu Yang,Maoqing Ye,Yingjun Chang,Weihua Liu,Xiaowo Wang,Zhenyu Ju,Jianwei Wang

View all publications

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IL27RA/WSX-1 抗体 [EPR20863-3] - BSA and Azide free