重组Anti-IL1 Receptor I/IL-1R-1抗体[EPR23149-131] - BSA and Azide free (Capture) (ab281217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23149-131] to IL1 Receptor I/IL-1R-1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Rat
概述
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产品名称
Anti-IL1 Receptor I/IL-1R-1抗体[EPR23149-131] - BSA and Azide free (Capture)
参阅全部 IL1 Receptor I/IL-1R-1 一抗 -
描述
兔单克隆抗体[EPR23149-131] to IL1 Receptor I/IL-1R-1 - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281217 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat IL1R1. The recommended pair for sandwich ELISA is:
Capture: ab281217, Rat IL1R1 Capture Antibody (unconjugated)
Detector: ab281067, Rat IL1R1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23149-131 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
- Anti-IL1 Receptor I/IL-1R-1 antibody [EPR23149-25] - BSA and Azide free (Capture) (ab281067)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23149-25] to IL1 Receptor I/IL-1R-1 - BSA and Azide free (Capture) (ab281067).
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23149-25] to IL1 Receptor I/IL-1R-1 - BSA and Azide free (Capture) (ab281067). |
靶标
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功能
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
序列相似性
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 25663 Rat
- SwissProt: Q02955 Rat
- Unigene: 9758 Rat
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别名
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281217 尚未被引用在任何文献中。