重组Anti-IL-9抗体[EPR23484-151] - BSA and Azide free (ab272699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23484-151] to IL-9 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-9抗体[EPR23484-151] - BSA and Azide free
参阅全部 IL-9 一抗 -
描述
兔单克隆抗体[EPR23484-151] to IL-9 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IP, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: His-tagged mouse IL9 recombinant protein, Mouse and rat spleen, thymus lysates. ICC/IF: 293T cell. Flow Cyt (intra): 293T cell. IP: Mouse spleen lysate.
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常规说明
ab272699 is the carrier-free version of ab227037.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23484-151 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272699于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. |
靶标
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功能
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
序列相似性
Belongs to the IL-7/IL-9 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3578 Human
- Entrez Gene: 16198 Mouse
- Entrez Gene: 116558 Rat
- Omim: 146931 Human
- SwissProt: P15248 Human
- SwissProt: P15247 Mouse
- Unigene: 960 Human
- Unigene: 3006 Mouse
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别名
- Cytokine P40 antibody
- Homolog of mouse T cell and mast cell growth factor 40 antibody
- HP40 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).
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IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10µg with ab227037 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10µg
Lane 2: ab227037 IP in mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227037 in mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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