Anti-IL-8抗体(ab7747)

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab7747 bisher noch nicht in Durchflusszytometrie getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab7747 abdecken, und gegen eine erneute Bestellung weitere Produkte von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab7747 in Durchflusszytometrie testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in Durchflusszytometrie.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie könnenweitere Produkte zu dem gleichen Preis wie ab7747 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab7747 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

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Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

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I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Thank you for your reply.

In theory it is possible to perform IHC-P with a directly conjugated antibody. This will also mean that the amplification that is achieve by adding the secondary is lost.

So my advise would be to only do it for abundant antigens.

ab7747 is not conjugated to HRP and therefore will need a secondary antibody that is conjugated.

I can recommend to use one of these:

ab98485

https://www.abcam.com/Goat-Fab2-Rabbit-IgG-Fc-HRP-pre-adsorbed-ab98485.html (or use the following: https://www.abcam.com/Goat-Fab2-Rabbit-IgG-Fc-HRP-pre-adsorbed-ab98485.html).

ab97085

https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-preadsorbed-ab97085.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-preadsorbed-ab97085.html).

We also offer EasyLink kits that can be used to conjugate this antibody directly to HRP (ab102892).

https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-10g-HRP-ab102892.html (or use the following: https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-10g-HRP-ab102892.html).

It would not be 100% optimal in this case since the antibody is only purified as IgG fraction.

I hope this information is helpful and wish you a good weekend.

Thank you for your inquiry.

I am happy to confirm that the recommended protocol in IHC is the following:

Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

Antibody incubation: 120 min, 20C 1:500

(http://www.ncbi.nlm.nih.gov/pubmed/17208399?dopt=Abstract)

I hope this information is helpful and wish you good luck with your experiments.

Thank you for your call today and for letting us know about the trouble with ab7747.

As we discussed, I'm sending a free of charge vial of ab18672 on the order *** which should arrive tomorrow.

Please keep me updated about the results using this new antibody. I look forward to hearing from you, and let me know if you have any questions or if there is anything else that we can do for you.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Thank you for your protocol, I have looked at it in details and I would like to suggest trying the following changes to see if you start having a cytoplasmic staining rather than a nuclear staining. -check you are not overfixing (or underfixing) the tissue, different fixation times could be tested to see if this may be a problem -try a different antigen retrieval method, such as enzymatic. This way if the citrate buffer antigen retrieval method is damaging the epitope (and other proteins) you may see a difference with a different antigen retrieval step -check with a no primary control that the staining is not due to the secondary or other further steps in the staining protocol -try blocking peroxidases with only 0.3% 10-20min, too much peroxidase can damage the tissue. -remove the horse serum and use only 10% normal goat serum to block the tissue (just in case the serum is giving the problem) I think in general your protocol is very good and hope that the above suggestions will help. If you want to be certain of the staining I would recommend to run another IL8 antibody along the sister sections to see if it gives you the same staining pattern. As ab7747 has not been tested in IHC it is hard to be sure the problem is not that the antibody does not work in IHC.