Anti-IL-6R抗体(ab128008)
Key features and details
- Rabbit polyclonal to IL-6R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IL-6R抗体
参阅全部 IL-6R 一抗 -
描述
兔多克隆抗体to IL-6R -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IL-6R aa 253-267.
Database link: P08887 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
(without Mg2+, Ca2+) -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128008于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/500. Predicted molecular weight: 52 kDa.
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IHC-P | (2) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/200 - 1/500. Predicted molecular weight: 52 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
组织特异性
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
结构域
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
翻译后修饰
A short soluble form may also be released from the membrane by proteolysis. -
细胞定位
Secreted and Basolateral cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3570 Human
- Omim: 147880 Human
- SwissProt: P08887 Human
- Unigene: 709210 Human
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别名
- CD 126 antibody
- CD126 antibody
- CD126 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody (ab128008)
Paraffin-embedded human spleen tissue stained for IL6R with ab128008 at 2.5 µg/ml in immunohistochemical analysis.
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All lanes : Anti-IL-6R antibody (ab128008) at 1/200 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : 803 cell lysate
Predicted band size: 52 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab128008 被引用在 28 文献中.
- Chung AW et al. Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6. NPJ Breast Cancer 8:30 (2022). PubMed: 35260569
- Masalha M et al. IL6R is a target of miR-197 in human keratinocytes. Exp Dermatol 30:1177-1186 (2021). PubMed: 32780449
- Shi C et al. lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93. Mediators Inflamm 2021:5318369 (2021). PubMed: 33505213
- Montgomery A et al. Overlapping and distinct biological effects of IL-6 classic and trans-signaling in vascular endothelial cells. Am J Physiol Cell Physiol 320:C554-C565 (2021). PubMed: 33471622
- Wiegertjes R et al. Increased IL-6 receptor expression and signaling in ageing cartilage can be explained by loss of TGF-β-mediated IL-6 receptor suppression. Osteoarthritis Cartilage 29:773-782 (2021). PubMed: 33617971