Anti-IL-6抗体[1.2-2B11-2G10] (ab9324)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.cn/ps/products/9/ab9324/Images/ab9324-454107-anti-il-6-antibody-immunohistochemistry-liver-tumor-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)")
Key features and details
- Mouse monoclonal [1.2-2B11-2G10] to IL-6
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-IL-6抗体[1.2-2B11-2G10]
参阅全部 IL-6 一抗 -
描述
小鼠单克隆抗体[1.2-2B11-2G10] to IL-6 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Sandwich ELISA, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
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阳性对照
- IHC-P: Human liver tumor and prostate tumor tissues; WB: Human spleen and lung tissue lysates, Rat spleen and lung tissue lysates, Recombinant human IL6 protein (ab9627).
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常规说明
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1.2-2B11-2G10 -
同种型
IgG2a -
研究领域
相关产品
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (12) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Sandwich ELISA |
Use at an assay dependent concentration.
In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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| WB | (3) |
Use at an assay dependent concentration.
To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
| 说明 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Sandwich ELISA
Use at an assay dependent concentration. In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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WB
Use at an assay dependent concentration. To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
靶标
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功能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
疾病相关
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
序列相似性
Belongs to the IL-6 superfamily. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3569 Human
- Entrez Gene: 24498 Rat
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P20607 Rat
- Unigene: 654458 Human
- Unigene: 9873 Rat
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别名
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.cn/ps/products/9/ab9324/Images/ab9324-251202-anti-il-6-antibody-western-blot.jpg)
Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)All lanes : Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) at 1/2000 dilution
Lane 1 : Human Spleen
Lane 2 : Human Lung
Lane 3 : Rat Spleen
Lane 4 : Rat Lung
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : 800CW Goat Anti-Mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17,25 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible multimer)This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.cn/ps/products/9/ab9324/Images/ab9324-454110-anti-il-6-antibody-immunohistochemistry-prostate-tumor-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.cn/ps/products/9/ab9324/Images/ab9324-400102-Western-blot--AntiIL6-antibody-ab9324.png)
Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)ab9324 detecting IL-6 by sandwich ELISA.
数据表及文件
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Datasheet download
文献 (320)
ab9324 被引用在 320 文献中.
- Najjar RS et al. Raspberry polyphenols target molecular pathways of heart failure. J Nutr Biochem 124:109535 (2024). PubMed: 37984734
- Song Q et al. Nonmetastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Patients with Breast Cancer at Risk for Metastasis. Am J Pathol 194:253-263 (2024). PubMed: 38029922
- Cho KH et al. Enhancement of Antioxidant and Anti-Glycation Properties of Beeswax Alcohol in Reconstituted High-Density Lipoprotein: Safeguarding against Carboxymethyllysine Toxicity in Zebrafish. Antioxidants (Basel) 12:N/A (2023). PubMed: 38136235
- Han H et al. Long Non-Coding RNA LOC339059 Attenuates IL-6/STAT3-Signaling-Mediated PDL1 Expression and Macrophage M2 Polarization by Interacting with c-Myc in Gastric Cancer. Cancers (Basel) 15:N/A (2023). PubMed: 38001573
- Yalcin A et al. Protective effect of melatonin on learning and memory impairment and hippocampal dysfunction in rats induced by high-fructose corn syrup. Iran J Basic Med Sci 26:69-75 (2023). PubMed: 36594054
