重组Anti-IL-4抗体[EPR1118Y] (ab62351)
![Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.cn/ps/products/62/ab62351/Images/ab62351-275012-anti-il-4-antibody-epr1118y-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1118Y] to IL-4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-4抗体[EPR1118Y]
参阅全部 IL-4 一抗 -
描述
兔单克隆抗体[EPR1118Y] to IL-4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Human tonsil lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1118Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
|
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| ICC/IF |
1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa. |
|
ICC/IF
1/500. |
靶标
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功能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
疾病相关
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
序列相似性
Belongs to the IL-4/IL-13 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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别名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)
Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.cn/ps/products/62/ab62351/Images/ab62351-262046-anti-il-4-antibody-epr1118y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.cn/ps/products/62/ab62351/Images/ab62351-75666-anti-il-4-antibody-epr1118y-western-blot.jpg)
Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
![Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.cn/ps/products/62/ab62351/Images/ab62351-4-benefits-of-recombinant-antibodies.png)
Anti-IL-4 antibody [EPR1118Y] (ab62351)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab62351 被引用在 11 文献中.
- Liu G et al. A novel inflammation-associated prognostic signature for clear cell renal cell carcinoma. Oncol Lett 24:307 (2022). PubMed: 35949606
- Almeida AF et al. Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles. Int J Mol Sci 23:N/A (2022). PubMed: 36499452
- Jin Y & Zhang A Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7-TGF-ß pathway in allergic rhinitis. Mol Med Rep 25:N/A (2022). PubMed: 35029288
- Yu HZ et al. NR4A1 agonist cytosporone B attenuates neuroinflammation in a mouse model of multiple sclerosis. Neural Regen Res 17:2765-2770 (2022). PubMed: 35662227
- Becares N et al. Immune Regulatory Mediators in Plasma from Patients With Acute Decompensation Are Associated With 3-Month Mortality. Clin Gastroenterol Hepatol 18:1207-1215.e6 (2020). PubMed: 31446184