Anti-IL-3RB (phospho Y593)抗体(ab53695)
Key features and details
- Rabbit polyclonal to IL-3RB (phospho Y593)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-3RB (phospho Y593)抗体
参阅全部 IL-3RB 一抗 -
描述
兔多克隆抗体to IL-3RB (phospho Y593) -
宿主
Rabbit -
特异性
ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human IL-3RB aa 550-650 (phospho Y593).
Database link: P32927 -
阳性对照
- Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
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| 说明 |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 97 kDa. |
靶标
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功能
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor. -
序列相似性
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1439 Human
- Entrez Gene: 12983 Mouse
- Omim: 138981 Human
- SwissProt: P32927 Human
- SwissProt: P26955 Mouse
- Unigene: 592192 Human
- Unigene: 235324 Mouse
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别名
- Beta-C antibody
- CD131 antibody
- CDw131 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-3RB (phospho Y593) antibody (ab53695)ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-IL-3RB (phospho Y593) antibody (ab53695)All lanes : Anti-IL-3RB (phospho Y593) antibody (ab53695)
Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes)
Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide
Predicted band size: 97 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab53695 尚未被引用在任何文献中。