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Anti-IL-34抗体(ab224734)

Submit a review Submit a question References (4)
Western blot - Anti-IL-34 antibody (ab224734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-34 antibody (ab224734)

Key features and details

  • Rabbit polyclonal to IL-34
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-IL-34抗体
    参阅全部 IL-34 一抗

  • 描述

    兔多克隆抗体to IL-34

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human IL-34 aa 191-240.
    Database link: Q6ZMJ4

  • 阳性对照

    • WB: PC-3 and HepG2 whole cell lysate (ab7900). IHC-P: Human small intestine tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab224734于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/20 - 1/200.
WB
1/500 - 1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
说明
IHC-P
1/20 - 1/200.
WB
1/500 - 1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能

    Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.

  • 组织特异性

    Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

  • 序列相似性

    Belongs to the IL-34 family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • C16orf77 antibody
      • Chromosome 16 open reading frame 77 antibody
      • FPT025 antibody
      • IL 34 antibody
      • IL-34 antibody
      • Il34 antibody
      • IL34_HUMAN antibody
      • Interleukin 34 antibody
      • Interleukin-34 antibody
      • Interleukin34 antibody
      • MGC34647 antibody
      see all

    图片

    • Western blot - Anti-IL-34 antibody (ab224734)
      Western blot - Anti-IL-34 antibody (ab224734)
      All lanes : Anti-IL-34 antibody (ab224734) at 1/500 dilution

      Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 27 kDa
      Observed band size: 27 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-34 antibody (ab224734)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-34 antibody (ab224734)

      Paraffin-embedded human small intestine tissue stained for IL-34 with ab224734 at 1/100 dilution in immunohistochemical analysis.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab224734？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab224734 被引用在 4 文献中.

    • Hu Y  et al. Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer. Front Oncol 12:845765 (2022). PubMed: 35936748
    • Wang J  et al. Interleukin-34 accelerates intrauterine adhesions progress related to CX3CR1+ monocytes/macrophages. Eur J Immunol 51:2501-2512 (2021). PubMed: 34138470
    • Wang Z  et al. Loss of IL-34 Expression Indicates Poor Prognosis in Patients With Lung Adenocarcinoma. Front Oncol 11:639724 (2021). PubMed: 34336646
    • de Vries WM  et al. M-CSF and IL-34 expression as indicators for growth in sporadic vestibular schwannoma. Virchows Arch N/A:N/A (2018). PubMed: 30580386

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