Anti-IL-33抗体[Nessy-1] - BSA and Azide free (ab54385)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [Nessy-1] - BSA and Azide free (ab54385)](https://www.abcam.cn/ps/products/54/ab54385/Images/ab54385-411538-AntiIL33-antibody.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [Nessy-1] - BSA and Azide free (ab54385)")
Key features and details
- Mouse monoclonal [Nessy-1] to IL-33 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IL-33抗体[Nessy-1] - BSA and Azide free
参阅全部 IL-33 一抗 -
描述
小鼠单克隆抗体[Nessy-1] to IL-33 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human IL-33 aa 112-270.
Database link: O95760 -
阳性对照
- IHC: Human stomach tissue WB: Liver lysate
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常规说明
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
Nessy-1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
组织特异性
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
序列相似性
Belongs to the IL-1 family. Highly divergent. -
结构域
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
翻译后修饰
The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
细胞定位
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
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数据库链接
- Entrez Gene: 90865 Human
- Omim: 608678 Human
- SwissProt: O95760 Human
- Unigene: 731660 Human
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别名
- C9orf26 antibody
- CHROMOSOME 9 OPEN READING FRAME 26 antibody
- DKFZp586H0523 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [Nessy-1] - BSA and Azide free (ab54385)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human stomach tissue labelling IL-33 with ab54358 at 10 µg/mL.
数据表及文件
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Datasheet download
文献 (23)
ab54385 被引用在 23 文献中.
- Jin R et al. The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia. Lab Invest 102:794-804 (2022). PubMed: 35306530
- Chen L et al. Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor. Elife 11:N/A (2022). PubMed: 35998104
- Zhang B et al. Hepatic progenitor cells promote the repair of schistosomiasis liver injury by inhibiting IL-33 secretion in mice. Stem Cell Res Ther 12:546 (2021). PubMed: 34674752
- Meng L et al. Characteristics of the Gut Microbiome and IL-13/TGF-β1 Mediated Fibrosis in Post-Kasai Cholangitis of Biliary Atresia. Front Pediatr 9:751204 (2021). PubMed: 34858903
- Wang S et al. The Effects of Interleukin-33 (IL-33) on Osteosarcoma Cell Viability, Apoptosis, and Epithelial-Mesenchymal Transition are Mediated Through the PI3K/AKT Pathway. Med Sci Monit 26:e920766 (2020). PubMed: 32312946
