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重组RabMAb

重组Anti-IL-33抗体[EPR20417] (ab207737)

Submit a review Submit a question References (11)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
  • Anti-IL-33 antibody [EPR20417] (ab207737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20417] to IL-33
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-IL-33抗体[EPR20417]
    参阅全部 IL-33 一抗

  • 描述

    兔单克隆抗体[EPR20417] to IL-33

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human IL33 recombinant protein. THP-1 treated with Phorbol-12-myristate-13-acetate and LPS lysate. Human artery, lung, tonsil, stomach and colon lysate. His-tagged rat IL33 recombinant protein. Rat spleen, kidney and lung lysate. IHC-P: Rat lung and spleen tissue. Human tonsil tissue and kidney cancer tissue. ICC/IF: THP-1 cells.

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab207737于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Predicted molecular weight: 30 kDa.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Under our testing conditions we observed non-specific staining in some human tissues, customers are recommended to optimize IHC testing conditions.
说明
WB
1/1000. Predicted molecular weight: 30 kDa.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Under our testing conditions we observed non-specific staining in some human tissues, customers are recommended to optimize IHC testing conditions.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).

  • 组织特异性

    Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

  • 序列相似性

    Belongs to the IL-1 family. Highly divergent.

  • 结构域

    The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.

  • 翻译后修饰

    The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.

  • 细胞定位

    Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt

  • 数据库链接

    • 别名

      • C9orf26 antibody
      • CHROMOSOME 9 OPEN READING FRAME 26 antibody
      • DKFZp586H0523 antibody
      • DVS27 antibody
      • DVS27 related protein antibody
      • IL 1F11 antibody
      • IL 33 antibody
      • IL-1F11 antibody
      • IL-33 antibody
      • IL1F11 antibody
      • IL33 antibody
      • IL33_HUMAN antibody
      • Interleukin 1 family member 11 antibody
      • Interleukin 33 antibody
      • INTERLEUKIN 33 NFHEV antibody
      • Interleukin 33 precursor antibody
      • Interleukin-1 family member 11 antibody
      • Interleukin-33 (109-270) antibody
      • Interleukin33 antibody
      • NF HEV antibody
      • NF-HEV antibody
      • NFEHEV antibody
      • NFHEV antibody
      • Nuclear factor for high endothelial venules antibody
      • Nuclear factor from high endothelial venules antibody
      • OTTHUMP00000021041 antibody
      • RP11 575C20.2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

      Immunohistochemical analysis of paraffin embedded rat spleen tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelium cells of rat spleen (PMID: 12819012, PMID: 18836528) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution + human IL33 recombinant protein (aa112-270), 10 ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?


      Exposure time: 2 seconds


      Blocking: 5% NFDM/TBST

    • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate
      Lane 2 : THP-1 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharides (LPS) for 24 hours, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 30 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      The level of IL33 expression can be induced by PMA and LPS treatment (PMID: 19559631).

      Blocking : 5% NFDM/TBST

       

    • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

      Lane 1 : Human artery
      Lane 2 : Human lung
      Lane 3 : Human tonsil
      Lane 4 : Human stomach
      Lane 5 : Human colon

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?



      Blocking: 5% NFDM/TBST 

      Exposure times:

      Lanes 1-3,5: 3 minutes

      Lanes 4: 30 seconds

    • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution + Recombinant Rat IL-33 protein (ab207126)

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      Western blot - Anti-IL-33 antibody [EPR20417] (ab207737)
      All lanes : Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

      Lane 1 : Rat spleen
      Lane 2 : Rat kidney
      Lane 3 : Rat lung

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?



      Blocking: 5% NFDM/TBST 

      Exposure times:

      Lane 1: 3 seconds

      Lane 2: 10 seconds

      Lane3: 5 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

      Immunohistochemical analysis of paraffin embedded rat lung tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on epithelium and endothelium cells of rat lung (PMID: 18836528) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

      Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on high endothelial venules of human tonsil (PMID: 12819012) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (ab207737)

      Immunohistochemical analysis of paraffin embedded human kidney cancer tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelial cells of human kidney cancer (PMID: 12819012) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-IL-33 antibody [EPR20417] (ab207737)
      Anti-IL-33 antibody [EPR20417] (ab207737)

    文献 (11)

    发表研究结果有使用 ab207737？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab207737 被引用在 11 文献中.

    • Fan Y  et al. Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury. Nat Commun 14:4796 (2023). PubMed: 37558705
    • Zhang Y  et al. Relationship and prognostic significance of IL-33, PD-1/PD-L1, and tertiary lymphoid structures in cervical cancer. J Leukoc Biol 112:1591-1603 (2022). PubMed: 35501298
    • Wu Q  et al. A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats. J Mol Neurosci 72:730-740 (2022). PubMed: 34988900
    • Goda N  et al. The Effect of Intratumoral Interrelation among FOXP3+ Regulatory T Cells on Treatment Response and Survival in Triple-Negative Breast Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35565267
    • Widjaja AA  et al. IL11 Stimulates IL33 Expression and Proinflammatory Fibroblast Activation across Tissues. Int J Mol Sci 23:N/A (2022). PubMed: 36012165
    View all Publications for this product

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