Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-IL-31抗体(ab37157)

Submit a review Q&A (1)References (1)
Western blot - Anti-IL-31 antibody (ab37157)

    Key features and details

    • Rabbit polyclonal to IL-31
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    概述

    • 产品名称

      Anti-IL-31抗体
      参阅全部 IL-31 一抗

    • 描述

      兔多克隆抗体to IL-31

    • 宿主

      Rabbit

    • 特异性

      ab37157 recognises Interleukin 31 (IL31).

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Synthetic peptide corresponding to Mouse IL-31 (N terminal).
      (Peptide available as ab39776)

    • 阳性对照

      • WB: RAW264.7 cell lysate

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab37157于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).
    说明
    WB
    Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).

    靶标

    • 功能

      Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

    • 组织特异性

      Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 数据库链接

      • 形式

        Amino acids 1-23 are a signal peptide.

      • 别名

        • IL 31 antibody
        • IL-31 antibody
        • IL31 antibody
        • IL31_HUMAN antibody
        • Interleukin 31 antibody
        • Interleukin-31 antibody
        see all

      图片

      • Western blot - Anti-IL-31 antibody (ab37157)
        Western blot - Anti-IL-31 antibody (ab37157)
        Lane 1 : Anti-IL-31 antibody (ab37157) at 2.5 µg/ml
        Lane 2 : Anti-IL-31 antibody (ab37157) at 5 µg/ml
        Lane 3 : Anti-IL-31 antibody (ab37157) at 10 µg/ml

        All lanes : RAW264.7 cell lysate

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 18 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?

      文献 (1)

      发表研究结果有使用 ab37157？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab37157 被引用在 1 文献中.

      • Niyonsaba F  et al. Antimicrobial peptides human beta-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine IL-31 by human mast cells. J Immunol 184:3526-34 (2010). PubMed: 20190140

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      Hi,



      I was searching for IL31 antibody. This product (ab37157) is interesting to me. But I have a question regarding this product.



      In the data sheet of this antibody, there was a picture showing bands in 3 lanes with different concentration of the antibody. How can you do the western blot on one membrane using different concentration of antibody? Is there any new technology or just a mistake?

      Read More
      Answer

      Thank you for contacting us. The image was labelled incorrectly, all lanes were blotted with a concentration of antobody 10 ug/ml. We are updating the datasheet now, thank you for bringing this error to our attention.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com