AB250239

重组Anti-IL-25抗体[EPR5743(2)] - BSA and Azide free

Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal IL-25 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples.

查看别名

IL17E, UNQ3120/PRO10272, IL25, Interleukin-25, IL-25, Interleukin-17E, IL-17E

1 Images

Supplier Data

Western blot - Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free (AB250239)

This data was developed using ab180594, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IL-25 antibody [EPR5743(2)] (<a href='/products/primary-antibodies/il-25-antibody-epr57432-ab180594'>ab180594</a>) at 1/5000 dilution

All lanes:

Human IL-17E recombinant protein aa1-145 at 0.05 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 16 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-IL-25 antibody [EPR5743(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5743(2)

亚型

IgG

不含载体蛋白

Yes

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

ab250239 is the carrier-free version of ab180594.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production (PubMed : 15860795, PubMed : 25821217). Promotes and augments T-helper type 2 responses locally or systemically (PubMed : 25821217). Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13 (PubMed : 25821217). Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways (PubMed : 15860795). Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13 (PubMed : 11754819).

See full target information IL25

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-IL-25抗体[EPR5743(2)] - BSA and Azide free