重组Anti-IL-2 Receptor alpha抗体[EPR6452] (ab128955)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-253764-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6452] to IL-2 Receptor alpha
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-2 Receptor alpha抗体[EPR6452]
参阅全部 IL-2 Receptor alpha 一抗 -
描述
兔单克隆抗体[EPR6452] to IL-2 Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human IL-2 Receptor alpha aa 1-250.
Database link: P01589 -
阳性对照
- IHC-P: Hodgkin's lymphoma, human tonsil, spleen, liver, and thymus tissues. ICC/IF: Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6452 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128955于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
靶标
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功能
Receptor for interleukin-2. -
疾病相关
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
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别名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Immunohistochemical staining of paraffin embedded human Hodgkin lymphoma with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-253792-anti-il-2-receptor-alpha-antibody-epr6452-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Immunofluorescence staining of Jurkat cells with purified ab128955 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab128955 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-142852-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Unpurified ab128955, at 1/250 dilution, staining IL2 Receptor alpha in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-253765-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-142851-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Unpurified ab128955 showing positive staining in Hodgkin's lymphoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-142850-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Unpurified ab128955 showing negative staining in Human liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-142849-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Unpurified ab128955 showing positive staining in Human thymus tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-142848-anti-il-2-receptor-alpha-antibody-epr6452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Unpurified ab128955 showing positive staining in Human spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-11-anti-il-2-receptor-alpha-antibody-epr6452-tissue-microarray-human-ab128955.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)Tissue Microarrays stained for "Anti-IL-2 Receptor alpha antibody [EPR6452]” using "ab128955" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab128955 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)](https://www.abcam.cn/ps/products/128/ab128955/Images/ab128955-9-benefits-of-recombinant-antibodies.png)
Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab128955 被引用在 15 文献中.
- Kemper K et al. Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models. Life Sci Alliance 5:N/A (2022). PubMed: 36271507
- Zadka Ł et al. Interplay of stromal tumor-infiltrating lymphocytes, normal colonic mucosa, cancer-associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer. Cancer Immunol Immunother 70:2681-2700 (2021). PubMed: 33625532
- Liang Q et al. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Cell Death Dis 12:336 (2021). PubMed: 33795653
- Gong L et al. CD4+CD25+ Regulatory T Cells in Intracranial Thrombi Are Inversely Correlated with Hemorrhagic Transformation after Thrombectomy: A Clinical-Immunohistochemical Analysis of Acute Ischemic Stroke. Oxid Med Cell Longev 2021:3143248 (2021). PubMed: 34055193
- Orlovic Vlaho M et al. CD25+ FOXP3+ and CD4+ CD25+ cells distribution in decidual departments of women with severe and mild pre-eclampsia: Comparison with healthy pregnancies. Am J Reprod Immunol 84:e13281 (2020). PubMed: 32485016