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重组RabMAb

重组Anti-IL-2抗体[RM1124] - BSA and Azide free (ab317332)

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Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
  • Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1124] to IL-2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Indirect ELISA
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-IL-2抗体[RM1124] - BSA and Azide free
    参阅全部 IL-2 一抗

  • 描述

    兔重组multiclonal [RM1124] to IL-2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, IP, Indirect ELISAmore details
    不适用于: Flow Cyt (Intra) or IHC-P

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Jurkat treated with 15nM 24h PMA and 1µM ionomycin and 300ng/ml BFA for 24 hours whole cell lysate. ICC/IF: Jurkat and EL4 cells. IP: Jurkat cell.

  • 常规说明

    ab317332 is the carrier-free version of ab317331

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317332于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra) or IHC-P.

靶标

  • 功能

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

  • 疾病相关

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

  • 序列相似性

    Belongs to the IL-2 family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • interleukin2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    图片

    • Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      All lanes : Anti-IL-2 antibody [RM1124] (ab317331) at 1/1000 dilution

      Lane 1 : Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
      Lane 2 : Jurkat treated with 15nM 24h PMA (ab120297) and 1µM ionomycin (ab120116) and 300ng/ml BFA for 24 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 17 kDa
      Observed band size: 15 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression of IL-2  is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      All lanes : Anti-IL-2 antibody [RM1124] (ab317331) at 1/1000 dilution

      Lane 1 : Untreated EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate
      Lane 2 : EL4.IL-2 cell line treated with Cell Stimulation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 17 kDa


      Exposure time: 103 seconds


      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression of IL-2  is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).

      The identity of the higher MW bands at approximately 50kDa are unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling IL-2 with ab317331 at 1/50 (10.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

      Confocal image showing cytoplasmic staining in jurkat cell line (shown in green) treated with 12-O-Tetradecanoylphorbol-13-acetate (15 nM), ionomycin (1 µm) and Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Left Panel is applied with Anti-IL-2 antibody [RM1024] (ab317331) at 1/50 dilution (10 ug/ml); Right panel is applied with Anti-IL-2 antibody (ab9618) at 1/100 dilution (10 ug/ml).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-2 with ab317331 at 1/50 (10.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

      Confocal image showing cytoplasmic staining in EL4 cells (shown in green) treated with TPA (40 nM), A23187 (2 uM) and BFA (300 ng/ml) for 23 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      IL-2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate with ab317331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
      Lane 2: ab317331 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317331 in Jurkat whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds.

    • Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

      This data was developed using ab317331, the same antibody clone in a different buffer formulation.

      Indirect ELISA analysis of ab317331 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

      Antigen: Human IL-2,Mouse IL-2.

      Antigen concentration: 1000 ng/ml

    • Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
      Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317332？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317332 尚未被引用在任何文献中。

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