Anti-IL-2抗体(ab180780)
Key features and details
- Rabbit polyclonal to IL-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-2抗体
参阅全部 IL-2 一抗 -
描述
兔多克隆抗体to IL-2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-2 aa 1-153.
Sequence:MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINN YKNPKLTRML TFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNF HLRPRDLISNINVIVLELKGSE TTFMCEYADETATIVEFLNRWITFCQ SIISTLT
Database link: P60568 -
阳性对照
- WB: Jurkat cells treated by TPA (40 nM), Calcimycin (2µM) and Brefeldin A (300 ng / ml) for 0-24 hours
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180780于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000. Predicted molecular weight: 17 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 17 kDa. |
靶标
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功能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
疾病相关
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
序列相似性
Belongs to the IL-2 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
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别名
- Aldesleukin antibody
- IL 2 antibody
- IL-2 antibody
see all
图片
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Western blot - Anti-IL-2 antibody (ab180780)All lanes : Anti-IL-2 antibody (ab180780) at 1/1000 dilution
Lane 1 : Jurkat cells untreated
Lane 2 : Jurkat cells treated by TPA (40 nM), Calcimycin (2µM) and Brefeldin A (300 ng / ml) for 0-24 hours
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 17 kDa
Exposure time: 180 secondsBlocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab180780 被引用在 12 文献中.
- Ohana J et al. Transformation of immunosuppressive mtKRAS tumors into immunostimulatory tumors by Nerofe and Doxorubicin. Oncotarget 14:688-699 (2023). PubMed: 37395796
- Mazumdar S et al. Immunomodulatory activity of ethanol extract of Annona reticulata L. leaf in cultured immune cells and in Swiss albino mice. J Ayurveda Integr Med 13:100554 (2022). PubMed: 35334452
- Baraya YS et al. Strobilanthes crispus elicits anti-tumor immunogenicity in in vitro and in vivo metastatic breast carcinoma. PLoS One 17:e0271203 (2022). PubMed: 35972917
- Chulpanova DS et al. Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer Cells. Biology (Basel) 10:N/A (2021). PubMed: 33579033
- Liang Z & Tang F The potency of lncRNA MALAT1/miR-155/CTLA4 axis in altering Th1/Th2 balance of asthma. Biosci Rep 40:N/A (2020). PubMed: 31909418