重组Anti-IL-18抗体[EPR19954] - BSA and Azide free (ab222926)
![Western blot - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-385129-anti-il-18-antibody-epr19954-bsa-and-azide-free-western-blot-human-lysate.jpg "Western blot - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19954] to IL-18 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-18抗体[EPR19954] - BSA and Azide free
参阅全部 IL-18 一抗 -
描述
兔单克隆抗体[EPR19954] to IL-18 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human IL-18 active protein; PC-3, Jurkat, A431, HeLa, HEK-293, and HaCaT cell lysates; Human skin and ovary cancer tissue lysates. Flow Cyt (intra): PC-3 and HaCaT cells.
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常规说明
ab222926 is the carrier-free version of ab207324.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19954 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
靶标
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功能
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
序列相似性
Belongs to the IL-1 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3606 Human
- Omim: 600953 Human
- SwissProt: Q14116 Human
- Unigene: 83077 Human
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别名
- Iboctadekin antibody
- IFN gamma inducing factor antibody
- IFN-gamma-inducing factor antibody
see all
图片
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Western blot - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)All lanes : Anti-IL-18 antibody [EPR19954] (ab207324) at 1/200 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IL18 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab207324).
Lanes 1-4: Merged signal (red and green). Green - ab207324 observed at 22 kDa. Red - loading control ab8245 observed at 37 kDa.
ab207324 Anti-IL-18 antibody [EPR19954] was shown to specifically react with IL-18 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265274 (knockout cell lysate ab256952) was used. Wild-type and IL-18 knockout samples were subjected to SDS-PAGE. ab207324 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 200 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-338338-anti-il-18-antibody-epr19954-western-blot.jpg)
Western blot - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)All lanes : Anti-IL-18 antibody [EPR19954] (ab207324) at 1/200 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : IL-18 knockout HEK-293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 22 kDaLanes 1 - 2: Merged signal (red and green). Green - ab207324 observed at 22 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab207324 was shown to recognize IL-18 in wild-type HEK293 cells as signal was lost at the expected MW in IL-18 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IL-18 knockout samples were subjected to SDS-PAGE. Ab207324 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207324).
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![Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-321020-anti-il-18-antibody-epr19954-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed PC-3 (Human prostate adenocarcinoma cell line) cells labeling IL-18with ab207324 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207324).
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![Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-321019-anti-il-18-antibody-epr19954-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HaCaT (Human keratinocyte cell line) cells labeling IL-18with ab207324 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207324).
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![Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-321018-anti-il-18-antibody-epr19954-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling IL-18with ab207324 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Negative control:
Jurkat cells serve as a negative cell line as described in PMID 15086390.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207324).
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![Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)](https://www.abcam.cn/ps/products/222/ab222926/Images/ab222926-6-benefits-of-recombinant-antibodies.png)
Anti-IL-18 antibody [EPR19954] - BSA and Azide free (ab222926)
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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