重组Anti-IL-17A抗体[RM1202] (ab318150)
![Western blot - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-545267-anti-il-17a-antibody-rm1202-western-blot-untreated-el4-mouse-lymphoma-t-lymphocyte-whole-cell-lysate-40-ug.jpg "Western blot - Anti-IL-17A antibody [RM1202] (ab318150)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1202] to IL-17A
- Suitable for: ICC/IF, Indirect ELISA, WB, IHC-P, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-IL-17A抗体[RM1202]
参阅全部 IL-17A 一抗 -
描述
兔重组multiclonal [RM1202] to IL-17A -
宿主
Rabbit -
特异性
This antibody does not cross-react with mouse IL-17F.Unsuitable for rat IHC-P.
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经测试应用
适用于: ICC/IF, Indirect ELISA, WB, IHC-P, IPmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours, whole cell lysate, Untreated NR8383 and NR8383 treated with 50ng/ml PMA, 500ng/ml lonomycin Ca2+Salt and 500ng/ml BFA overnight whole cell lysates. IHC-P: EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours cell pellet and untreated EL4 cell pellet. ICC/IF: EL4 cells. IP: EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
Recombinant Multiclonal -
克隆编号
RM1202 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318150于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50.
|
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| Indirect ELISA |
Use a concentration of 1 µg/ml.
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| WB |
1/1000. Predicted molecular weight: 18 kDa.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
|
| 说明 |
|---|
|
ICC/IF
1/50. |
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Indirect ELISA
Use a concentration of 1 µg/ml. |
|
WB
1/1000. Predicted molecular weight: 18 kDa. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
靶标
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功能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
组织特异性
Restricted to activated memory T-cells. -
序列相似性
Belongs to the IL-17 family. -
翻译后修饰
Found both in glycosylated and nonglycosylated forms. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 16171 Mouse
- Entrez Gene: 301289 Rat
- SwissProt: Q62386 Mouse
- SwissProt: Q61453 Rat
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别名
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
图片
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Western blot - Anti-IL-17A antibody [RM1202] (ab318150)All lanes : Anti-IL-17A antibody [RM1202] (ab318150) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 14-25 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Expression of IL-17A can be induced by PMA and Ionomycin treatment (PMID 28382171).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-545268-anti-il-17a-antibody-rm1202-western-blot-untreated-nr8383-rat-alveolar-macrophage-whole-cell-lysate-40-ug.jpg)
Western blot - Anti-IL-17A antibody [RM1202] (ab318150)All lanes : Anti-IL-17A antibody [RM1202] (ab318150) at 1/1000 dilution
Lane 1 : Untreated NR8383 (rat alveolar macrophage) whole cell lysate
Lane 2 : NR8383 treated with 50 ng/ml PMA, 500ng/ml lonomycin Ca2+Salt and 500ng/ml BFA overnight, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile is consistent with the literature (PMID: 17355969).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-4-anti-il-17a-antibody-rm1202-immunohistochemistry-a-el4-mouse-lymph.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [RM1202] (ab318150)Immunohistochemical analysis of paraffin-embedded (A) EL4 (mouse lymphoma T lymphocyte) treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours cell pellet. (B) Untreated EL4 cell pellet. tissue labeling IL-17A with ab318150 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining on (A) EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours cell pellet, weak staining on (B) untreated EL4 cell pellet. The section was incubated with ab318150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-5-anti-il-17a-antibody-rm1202-immunohistochemistry-mouse-skin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [RM1202] (ab318150)Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling IL-17A with ab318150 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Negative control: no staining on mouse skin. The section was incubated with ab318150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-3-anti-il-17a-antibody-rm1202-immunocytochemistry-el4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody [RM1202] (ab318150)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-17A with ab318150 at 1/50 (9.96 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing weak staining in EL4 cell line (shown in green). The expression increased after treatment with PMA (50 ng/ml), lonomycin Ca2 + Salt (500 ng/ml) and Brefeldin A (500 ng/ml) overnight (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Immunoprecipitation - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-6-anti-il-17a-antibody-rm1202-immunoprecipitation-el4.jpg)
Immunoprecipitation - Anti-IL-17A antibody [RM1202] (ab318150)IL-17A was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate with ab318150 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318150 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate
Lane 2: ab318150 IP in EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab318150 in treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 145 seconds.
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![Indirect ELISA - Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-7-anti-il-17a-antibody-rm1202-elisa-mouse-il-17amouse-il-17f.jpg)
Indirect ELISA - Anti-IL-17A antibody [RM1202] (ab318150)Indirect ELISA analysis of ab318150 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Mouse IL-17A,Mouse IL-17F.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with mouse IL-17F.
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![Anti-IL-17A antibody [RM1202] (ab318150)](https://www.abcam.cn/ps/products/318/ab318150/Images/ab318150-8-benefitsofrecombinantantibodies.jpg)
Anti-IL-17A antibody [RM1202] (ab318150)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318150 尚未被引用在任何文献中。