Anti-IL-13 receptor alpha 2 antibody [EPR29921-543]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type A375 (human malignant melanoma epithelial cell) cell pellet (B) IL13RA2 knockout A375 cell pellet tissue labeling IL-13 receptor alpha 2 with ab322898 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Wild-type A375 cell pellet, no staining on (B) IL13RA2 knockout A375 cell pellet.
The section was incubated with ab322898 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IL-13 receptor alpha 2 with ab322898 at 1/100 (4.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human skeletal muscle (HPA data).
The section was incubated with ab322898 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling IL-13 receptor alpha 2 with ab322898 at 1/100 (4.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human gastric adenocarcinoma.
The section was incubated with ab322898 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IL-13 receptor alpha 2 with ab322898 at 1/100 (4.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human colon.
The section was incubated with ab322898 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling IL-13 receptor alpha 2 with ab322898 at 1/100 (4.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human testis.
The section was incubated with ab322898 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (AB322898)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

In Western blot, ab322898 was shown to bind specifically to IL-13 receptor alpha 2. Band of interest was observed at 50-60 kDa in wild-type A375 cell lysate (lane 1) with no signal observed at this size in IL13RA2 knockout A375 cell lysate (lane 2) (lane 2, knockout cell line ab273381 / knockout cell lysate ab275532).

The molecular weight observed is consistent with what has been described in the literature (PMID : 30718742).

All lanes:

Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR29921-543] (ab322898) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

IL13RA2 knockout A375 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-60 kDa

true

Exposure time: 3s