Anti-IL-13 receptor alpha 1抗体[GM1E7] (ab39418)
Key features and details
- Mouse monoclonal [GM1E7] to IL-13 receptor alpha 1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-IL-13 receptor alpha 1抗体[GM1E7]
参阅全部 IL-13 receptor alpha 1 一抗 -
描述
小鼠单克隆抗体[GM1E7] to IL-13 receptor alpha 1 -
宿主
Mouse -
特异性
The antibody recognises IL-13 Receptor 1 alpha transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Other Immunogen Type corresponding to Human IL-13 receptor alpha 1. Genetic immunization with cDNA encoding human IL13-Rα1 (extracellular domain)
Database link: P78552 -
阳性对照
- ICC/IF: CHO cells transiently transfected with an expression vector encoding IL13-R. Flow Cyt: IL13-R transfected BOSC23 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
GM1E7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39418于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt | ||
ICC/IF |
说明 |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. -
组织特异性
Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells. -
序列相似性
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
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别名
- bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
- Cancer/testis antigen 19 antibody
- CD213a1 antibody
see all
图片
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Flow cytometric analysis of BOSC23 cells using ab39418. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ab39418 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
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Immunofluorescence staining of CHO cells using ab39418 at 1 µg/106 cells. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of ab39418 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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Specificity testing of ab39418. BOSC cells were transiently transfected with an expression vector encoding either the extracellular domain of IL-13Rα1 or an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody (B, green curves), an irrelevant monoclonal antibody served as negative control (bluecurves). For specificity testing (A), purified ab39418 was tested on both the IL-13Rα1 (red curve) and the irrelevant protein trans-fectants (green curve). A posi-tive signal was obtained only with IL-13Rα1 transfected cells.
数据表及文件
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Datasheet download
文献 (1)
ab39418 被引用在 1 文献中.
- Trüb M et al. Fibroblast activation protein-targeted-4-1BB ligand agonist amplifies effector functions of intratumoral T cells in human cancer. J Immunother Cancer 8:N/A (2020). PubMed: 32616554