重组Anti-IL-12B抗体[EPR5739] (ab133752)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5739] to IL-12B
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-12B抗体[EPR5739]
参阅全部 IL-12B 一抗 -
描述
兔单克隆抗体[EPR5739] to IL-12B -
宿主
Rabbit -
特异性
This antibody is unsuitable to detect endogenous IL-12B in WB.
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经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human IL-12B recombinant protein. ICC/IF: MOLT-4 cells Flow Cyt (intra): Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 4.30 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5739 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133752于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/300.
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
This antibody is unsuitable to detect endogenous IL12B in WB |
|
ICC/IF |
1/100 - 1/250.
|
说明 |
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Flow Cyt (Intra)
1/300. |
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). This antibody is unsuitable to detect endogenous IL12B in WB |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
疾病相关
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
翻译后修饰
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
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别名
- CLMF antibody
- CLMF p40 antibody
- CLMF2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified ab133752 at 1:100 dilution (3.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution (Purified)
All lanes : Human IL-12 recombinant protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.
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Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution + Human IL-12B recombinant protein at 0.01 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This image was generated using the unpurified version of the product.
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab133752 被引用在 6 文献中.
- Hildenbrand K et al. Human interleukin-12α and EBI3 are cytokines with anti-inflammatory functions. Sci Adv 9:eadg6874 (2023). PubMed: 37878703
- Mideksa YG et al. A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines. J Biol Chem 298:102677 (2022). PubMed: 36336075
- Bohnacker S et al. Influence of glycosylation on IL-12 family cytokine biogenesis and function. Mol Immunol 126:120-128 (2020). PubMed: 32823236
- Meier S et al. The molecular basis of chaperone-mediated interleukin 23 assembly control. Nat Commun 10:4121 (2019). PubMed: 31511508
- Reitberger S et al. Assembly-induced folding regulates interleukin 12 biogenesis and secretion. J Biol Chem 292:8073-8081 (2017). PubMed: 28325840
- Marks E et al. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation. Mucosal Immunol 10:1224-1236 (2017). PubMed: 28120851