重组Anti-IL-12A抗体[EP5737] (ab131039)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-260497-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5737] to IL-12A
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-12A抗体[EP5737]
参阅全部 IL-12A 一抗 -
描述
兔单克隆抗体[EP5737] to IL-12A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- NIH3T3, HuT-78, and Raw 264.7 cell lysates; Human kidney tissue, Human gastric carcinoma, Human colon tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP5737 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131039于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
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| IHC-P | (1) |
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
| Flow Cyt (Intra) |
1/19000.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
|
IHC-P
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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Flow Cyt (Intra)
1/19000. |
靶标
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
序列相似性
Belongs to the IL-6 superfamily. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3592 Human
- Entrez Gene: 16159 Mouse
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P43431 Mouse
- Unigene: 673 Human
- Unigene: 103783 Mouse
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别名
- CLMF antibody
- CLMF p35 antibody
- Cytotoxic lymphocyte maturation factor 1 p35 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)
Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL-12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-330898-anti-il-12a-antibody-ep5737-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)Intracellular Flow Cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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![Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-330897-anti-il-12a-antibody-ep5737-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)Intracellular Flow Cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1µg/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488® (Ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green).
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![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-260484-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution (purified) + HuT-78 (human Sezary syndrome) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-260483-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)All lanes : Anti-IL-12A antibody [EP5737] (ab131039) at 1/5000 dilution (purified)
Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-145254-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)All lanes : Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution (unpurified)
Lane 1 : NIH3T3 cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-145253-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL-12A with unpurified ab131039 at dilution of 1:250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-294303-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL-12A using ab131039 at 1/500 dilution in immunohistochemical analysis.
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![Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.cn/ps/products/131/ab131039/Images/ab131039-11-benefits-of-recombinant-antibodies.png)
Anti-IL-12A antibody [EP5737] (ab131039)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab131039 被引用在 11 文献中.
- Sánchez-Trujillo L et al. Chronic Venous Disease during Pregnancy Is Related to Inflammation of the Umbilical Cord: Role of Allograft Inflammatory Factor 1 (AIF-1) and Interleukins 10 (IL-10), IL-12 and IL-18. J Pers Med 13:N/A (2023). PubMed: 37373945
- Yu CR et al. Photoreceptor Cells Constitutively Express IL-35 and Promote Ocular Immune Privilege. Int J Mol Sci 23:N/A (2022). PubMed: 35897732
- Wan N et al. Tregs-derived interleukin 35 attenuates endothelial proliferation through STAT1 in pulmonary hypertension. Ann Transl Med 9:926 (2021). PubMed: 34350241
- Liu C et al. Avoiding Absolute Quantification Trap: A Novel Predictive Signature of Clinical Benefit to Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer. Front Immunol 12:782106 (2021). PubMed: 34868057
- Luchtel RA et al. High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model. Proc Natl Acad Sci U S A 117:1666-1677 (2020). PubMed: 31911474