AB131039

Anti-IL-12A 抗体 [EP5737]

Name: IL-12A抗体[EP5737] (ab131039)| Abcam中文官网
Brand: Abcam Limited
SKU: AB131039
Price: 2958 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-IL-12A antibody [EP5737]

RabMAb
Recombinant
了解详情

(1 Review)

(15 Publications)

Anti-IL-12A antibody [EP5737] (ab131039) is a rabbit monoclonal antibody detecting IL-12A in Western Blot, Flow Cytometry (Intra), IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL-12A with unpurified ab131039 at dilution of 1 : 250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (AB131039)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (AB131039)

Flow cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1© : g/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 © : g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488 (ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green)

IHC-P

AbReview52959****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL-12A using ab131039 at 1/500 dilution in immunohistochemical analysis.

This image is courtesy of an anonymous Abreview.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL-12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (AB131039)

Flow cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 © : g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

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Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution

All lanes:

HuT-78 (human Sezary syndrome) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 12 kDa,25 kDa

Observed band size: 35 kDa

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Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution

Lane 1:

NIH3T3 cell lysate at 10 µg

Lane 2:

HuT-78 cell lysate at 10 µg

Lane 3:

Raw 264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

false

Unknown

Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/5000 dilution

Lane 1:

NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

Lane 2:

Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 35 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-IL-12A antibody [EP5737] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP5737

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/19000", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/19000", "FlowCytIntra-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-IL-12A antibody [EP5737] (ab131039) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of IL-12A?
Anti-IL-12A [EP5737] (ab131039) specifically detects a band for IL-12A (UniProt: P29459) at a molecular weight of 25kDa.

Trusted by the scientific community
Anti-IL-12A [EP5737] (ab131039) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP5737] also available for your convenience: ab131039, Carrier free - ab214433, Carrier free - ab302861

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).

See full target information IL12A

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:6293 PubMed39984567

2025

IL35 modulates HBV-related HCC progression via IL6-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mingran Zhou,Yunhong Xia,Shuomin Wang

Current issues in molecular biology 46:3839-3865 PubMed38785507

2024

Prognostic Value of Histone Acetyl Transferase 1 (HAT-1) and Inflammatory Signatures in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Laura Jiménez-Álvarez,Oscar Fraile-Martinez,Cielo Garcia-Montero,Luis G Guijarro,Leonel Pekarek,Silvestra Barrena-Blázquez,Ángel Asúnsolo,Laura López-González,María Del Val Toledo-Lobo,Melchor Álvarez-Mon,Miguel A Saez,Alberto Gutiérrez-Calvo,Raúl Díaz-Pedrero

Journal of personalized medicine 13: PubMed37373945

2023

Chronic Venous Disease during Pregnancy Is Related to Inflammation of the Umbilical Cord: Role of Allograft Inflammatory Factor 1 (AIF-1) and Interleukins 10 (IL-10), IL-12 and IL-18.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Sánchez-Trujillo,Oscar Fraile-Martinez,Cielo García-Montero,Luis M García-Puente,Luis G Guijarro,Diego De Leon-Oliva,Diego Liviu Boaru,David Gardón-Alburquerque,María Del Val Toledo Lobo,Mar Royuela,Ignacio García-Tuñón,Antonio Rios-Parra,Juan A De León-Luis,Coral Bravo,Melchor Álvarez-Mon,Julia Bujan,Miguel A Saez,Natalio García-Honduvilla,Miguel A Ortega

Cell death discovery 9:67 PubMed36797242

2023

Interleukin-35 -producing B cells rescues inflammatory bowel disease in a mouse model via STAT3 phosphorylation and intestinal microbiota modification.

Applications

Unspecified application

Species

Unspecified reactive species

Minxiang Xie,Yuzhen Zhu,Yunjiao Zhou,Qiao Wang,Erli Gu,Yiwei Chu,Luman Wang

International journal of molecular sciences 23: PubMed35897732

2022

Photoreceptor Cells Constitutively Express IL-35 and Promote Ocular Immune Privilege.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Rong Yu,Manoj Kumar Yadav,Minkyung Kang,Yingyos Jittayasothorn,Lijin Dong,Charles E Egwuagu

Frontiers in immunology 12:782106 PubMed34868057

2021

Avoiding Absolute Quantification Trap: A Novel Predictive Signature of Clinical Benefit to Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengming Liu,Sihui Wang,Sufei Zheng,Fei Xu,Zheng Cao,Xiaoli Feng,Yan Wang,Qi Xue,Nan Sun,Jie He

Annals of translational medicine 9:926 PubMed34350241

2021

Tregs-derived interleukin 35 attenuates endothelial proliferation through STAT1 in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Naifu Wan,Wuwei Rong,Wentong Zhu,Daile Jia,Peiyuan Bai,Guizhu Liu,Qiangyou Wan,Ankang Lyu

European thyroid journal 9:139-147 PubMed32523890

2020

High Expression of Interleukin-12A and Its Association with the Clinicopathology and Prognosis of Differentiated Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Qiang Zhang,Chen-Tian Shen,Hong-Jun Song,Zhong-Ling Qiu,Quan-Yong Luo

EBioMedicine 52:102600 PubMed31981974

2020

Long non-coding RNA X-inactive specific transcript silencing ameliorates primary graft dysfunction following lung transplantation through microRNA-21-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Li,Li Wei,Zhijun Han,Zhong Chen,Quan Zhang

Proceedings of the National Academy of Sciences of 117:1666-1677 PubMed31911474

2020

High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model.

Applications

Unspecified application

Species

Unspecified reactive species