Anti-IL-12 p40抗体(ab62822)
Key features and details
- Goat polyclonal to IL-12 p40
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-12 p40抗体
参阅全部 IL-12 p40 一抗 -
描述
山羊多克隆抗体to IL-12 p40 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Cynomolgus monkey, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human IL-12 p40 aa 278-292 (internal sequence).
Sequence:C-QGKSKREKKDRVFTD
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阳性对照
- Recombinant Human IL-12 p80 protein (ab63226) can be used as a positive control in WB. Human Liver lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62822 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 4 - 6 µg/ml.
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| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
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IHC-P
Use a concentration of 4 - 6 µg/ml. |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
疾病相关
Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
翻译后修饰
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
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别名
- CLMF antibody
- CLMF p40 antibody
- CLMF2 antibody
see all
图片
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Western blot - Anti-IL-12 p40 antibody (ab62822)Anti-IL-12 p40 antibody (ab62822) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12 p40 antibody (ab62822)ab62822 at 4 µg/ml staining IL-12 p40 in Human colon tissue. Steamed antigen retrieval with citrate buffer pH 9 was performed, HRP-staining was used for detection.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62822 被引用在 1 文献中.
- Packer MS et al. Phage-assisted continuous evolution of proteases with altered substrate specificity. Nat Commun 8:956 (2017). PubMed: 29038472