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AB80682

Anti-IL-12抗体[AP-MAB0853]

Anti-IL-12 antibody [AP-MAB0853]

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(3 Publications)

Rat Monoclonal IL-12A antibody. Carrier free. Suitable for IP, Flow Cyt, WB, FuncS (Neut/Block) and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il12a.

查看别名

Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

AP-MAB0853

亚型

IgG1

不含载体蛋白

Yes

反应种属

Mouse

应用

Flow Cyt, IP, FuncS (Neut/Block), WB

applications

免疫原

Recombinant Fragment Protein within Mouse Il12a. The exact immunogen used to generate this antibody is proprietary information.

P43431

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>Neutralizes the bioactivity of natural or recombinant IL12 both in vivo and in vitro.</p>" } } }

产品详情

Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).

ab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 8766560). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (By similarity). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (PubMed : 18033300, PubMed : 30197594). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (By similarity).
See full target information Il12a

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Hepatology (Baltimore, Md.) 77:874-887 PubMed35592906

2023

Myeloid p38 activation maintains macrophage-liver crosstalk and BAT thermogenesis through IL-12-FGF21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

María Crespo,Ivana Nikolic,Alfonso Mora,Elena Rodríguez,Luis Leiva-Vega,Aránzazu Pintor-Chocano,Daniel Horrillo,Lourdes Hernández-Cosido,Jorge L Torres,Eva Novoa,Rubén Nogueiras,Gema Medina-Gómez,Miguel Marcos,Magdalena Leiva,Guadalupe Sabio

Infection and immunity 84:2802-12 PubMed27456834

2016

Proinflammatory M1 Macrophages Inhibit RANKL-Induced Osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuguno Yamaguchi,Alexandru Movila,Shinsuke Kataoka,Wichaya Wisitrasameewong,Montserrat Ruiz Torruella,Michiaki Murakoshi,Shinya Murakami,Toshihisa Kawai

Molecular medicine reports 12:7425-32 PubMed26459920

2015

Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zhou,Xiaoyong Li,Meina Feng,Qi Zhang,Zhendong Yang
View all publications

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