Anti-IL-12抗体(ab9992)
Key features and details
- Goat polyclonal to IL-12
- Suitable for: ELISA, IHC-P, Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: unknown
概述
-
产品名称
Anti-IL-12抗体
参阅全部 IL-12 一抗 -
描述
山羊多克隆抗体to IL-12 -
宿主
Goat -
经测试应用
适用于: ELISA, IHC-P, Sandwich ELISA, Neutralising, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human IL-12. p35: P29459 p40: P29460
-
阳性对照
- WB: Recombinant human IL-12 p40 protein (ab9993).
-
常规说明
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
IHC-P
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
靶标
-
功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
序列相似性
Belongs to the IL-6 superfamily. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161560 Human
- Omim: 161561 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
-
别名
- CLMF antibody
- CLMF p35 antibody
- CLMF p40 antibody
see all
数据表及文件
-
Datasheet download
文献 (9)
ab9992 被引用在 9 文献中.
- Zhang PP et al. COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy. Signal Transduct Target Ther 8:24 (2023). PubMed: 36609561
- Sandoval Pacheco CM et al. Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi. Parasite Immunol 45:e12971 (2023). PubMed: 36695719
- Jia H et al. NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion. Neuropathology 42:181-189 (2022). PubMed: 35434787
- Feng C et al. Single-cell RNA sequencing reveals distinct immunology profiles in human keloid. Front Immunol 13:940645 (2022). PubMed: 35990663
- Leng H et al. Effects of aloe polysaccharide, a polysaccharide extracted from Aloe vera, on TNF-a-induced HaCaT cell proliferation and the underlying mechanism in psoriasis. Mol Med Rep 18:3537-3543 (2018). PubMed: 30066937
- Kang H et al. Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathway. Int J Oncol 50:545-554 (2017). PubMed: 28101568
- Cousin C et al. The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation. Eur J Immunol 45:1772-82 (2015). PubMed: 25778793
- Al-Sammak F et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . PubMed: 24069393
- Schlaepfer E & Speck RF Anti-HIV activity mediated by natural killer and CD8+ cells after toll-like receptor 7/8 triggering. PLoS ONE 3:e1999 (2008). Neutralising ; Human . PubMed: 18431484
