Anti-IL-10RB/IL-10R2抗体[MM0360-8R24]

• Flow Cyt

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Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)

Overlay histogram showing Jurkat cells stained with ab89884 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89884, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• WB

Supplier Data

Western blot - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)

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Western blot - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (ab89884)

All lanes:

Human Placenta Tissue Lysate

Predicted band size: 36 kDa

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