重组Anti-IL-10抗体[EP1115Y] (ab52909)
![Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)](https://www.abcam.cn/ps/products/52/ab52909/Images/ab52909-65396-anti-il-10-antibody-ep1115y-western-blot.jpg "Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1115Y] to IL-10
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-10抗体[EP1115Y]
参阅全部 IL-10 一抗 -
描述
兔单克隆抗体[EP1115Y] to IL-10 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human IL-10 aa 100-200 (N terminal). The exact sequence is proprietary.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1115Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52909于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
This has only been tested against the recombinant protein. |
| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). This has only been tested against the recombinant protein. |
靶标
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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别名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
图片
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Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)Anti-IL-10 antibody [EP1115Y] (ab52909) at 1/5000 dilution + Recombinant protein (5ng)
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Anti-IL-10 antibody [EP1115Y] (ab52909)](https://www.abcam.cn/ps/products/52/ab52909/Images/ab52909-2-benefits-of-recombinant-antibodies.png)
Anti-IL-10 antibody [EP1115Y] (ab52909)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab52909 被引用在 3 文献中.
- Fitzpatrick CJ et al. Junin Virus Activates p38 MAPK and HSP27 Upon Entry. Front Cell Infect Microbiol 12:798978 (2022). PubMed: 35463647
- Abbasi M et al. Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments. Cureus 13:e13499 (2021). PubMed: 33777584
- de la Fuente C et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). PubMed: 29408900