Anti-IL-10抗体[B-S10] - BSA and Azide free (ab47222)
![Functional Studies - Anti-IL-10 antibody [B-S10] (ab47222)](https://www.abcam.cn/ps/products/47/ab47222/Images/ab47222-467752-AntiIL10-antibody-BS10functional-assay.jpg "Functional Studies - Anti-IL-10 antibody [B-S10] (ab47222)")
Key features and details
- Mouse monoclonal [B-S10] to IL-10 - BSA and Azide free
- Suitable for: Functional Studies
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IL-10抗体[B-S10] - BSA and Azide free
参阅全部 IL-10 一抗 -
描述
小鼠单克隆抗体[B-S10] to IL-10 - BSA and Azide free -
宿主
Mouse -
特异性
This antibody recognizes both natural and recombinant IL-10.
Blocks IL-10 induced inhibition of IL-6 synthesis on LPS activated monocytes.
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经测试应用
适用于: Functional Studiesmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-10.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
无载体
是 -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free. -
克隆
单克隆 -
克隆编号
B-S10 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
靶标
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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别名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
图片
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Functional Studies - Anti-IL-10 antibody [B-S10] (ab47222)
The isotype control antibody has no effect on IL-10 inhibition of IL-6 synthesis whilst the B-S10 antibody (anti-human IL-10) increases IL-6 secretion by PBMC activated LPS. B-S10 blocks the inhibition of IL-6 synthesis by IL-10 and increases IL-6 synthesis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab47222 被引用在 1 文献中.
- Bernatowicz PL et al. Synergistic effect of Dermatophagoides pteronyssinus allergens and dexamethasone on the expression of CD163 by peripheral blood mononuclear cells. Immunobiology 224:497-501 (2019). PubMed: 31133346
