Anti-IL-10抗体(ab34843)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: RIA, Neutralising, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IL-10抗体
参阅全部 IL-10 一抗 -
描述
兔多克隆抗体to IL-10 -
宿主
Rabbit -
特异性
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
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经测试应用
适用于: RIA, Neutralising, WB, ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Full length native protein (purified) corresponding to Human IL-10.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34843于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
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| Neutralising |
Use at an assay dependent concentration.
|
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| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
| ELISA |
Use at an assay dependent concentration.
(as detection antibody, using a monoclonal antibody for capture). |
| 说明 |
|---|
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RIA
Use at an assay dependent concentration. |
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Neutralising
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
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ELISA
Use at an assay dependent concentration. (as detection antibody, using a monoclonal antibody for capture). |
靶标
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功能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
组织特异性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
序列相似性
Belongs to the IL-10 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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别名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
图片
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Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein
Secondary
Goat anti-rabbit at 1/20000 dilution
Predicted band size: 21 kDa
4-20% reducing gel.Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.Dilution buffer: 3% BSA-TBS.Incubated O/N at 4°C. -
Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng
Secondary
Peroxidase conjugated secondary at 1/40000 dilution
Predicted band size: 21 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (91)
ab34843 被引用在 91 文献中.
- Sandoval Pacheco CM et al. Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi. Parasite Immunol 45:e12971 (2023). PubMed: 36695719
- Gál L et al. Restoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury. Research (Wash D C) 6:0056 (2023). PubMed: 36930811
- Xie Q et al. Chitosan oligosaccharide improves ovarian granulosa cells inflammation and oxidative stress in patients with polycystic ovary syndrome. Front Immunol 14:1086232 (2023). PubMed: 36936973
- Lvova MN et al. Curcumin and Its Supramolecular Complex with Disodium Glycyrrhizinate as Potential Drugs for the Liver Fluke Infection Caused by Opisthorchis felineus. Pathogens 12:N/A (2023). PubMed: 37375509
- Moyano A et al. EBV Impact in Peripheral Macrophages' Polarization Cytokines in Pediatric Patients. Viruses 15:N/A (2023). PubMed: 37896882