Anti-IL-1 beta抗体(ab9787)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-IL-1 beta抗体
参阅全部 IL-1 beta 一抗 -
描述
兔多克隆抗体to IL-1 beta -
宿主
Rabbit -
特异性
This antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected. The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein. -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Rat IL-1 beta aa 1 to the C-terminus.
Database link: Q63264 -
阳性对照
- Recombinant rat IL-1 beta (lower limit of detection: 1.95 ng/lane)
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常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a ab245863 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta. |
说明 |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a ab245863 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta. |
靶标
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功能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
组织特异性
Expressed in activated monocytes/macrophages (at protein level). -
序列相似性
Belongs to the IL-1 family. -
翻译后修饰
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
细胞定位
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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别名
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (87)
ab9787 被引用在 87 文献中.
- Malek N et al. Description of Novel Molecular Factors in Lumbar DRGs and Spinal Cord Factors Underlying Development of Neuropathic Pain Component in the Animal Model of Osteoarthritis. Mol Neurobiol 61:1580-1592 (2024). PubMed: 37731080
- Sun F et al. Allicin protects against LPS-induced cardiomyocyte injury by activating Nrf2-HO-1 and inhibiting NLRP3 pathways. BMC Cardiovasc Disord 23:410 (2023). PubMed: 37596540
- Zhou Y et al. Receptor-interacting protein 1 inhibition prevents mechanical stress-induced temporomandibular joint osteoarthritis by regulating apoptosis and later-stage necroptosis of chondrocytes. Arch Oral Biol 147:105612 (2023). PubMed: 36603515
- Lan T et al. PPAR-γ activation promotes xenogenic bioroot regeneration by attenuating the xenograft induced-oxidative stress. Int J Oral Sci 15:10 (2023). PubMed: 36797252
- Bechinger P et al. In-vivo time course of organ uptake and blood-brain-barrier permeation of poly(L-lactide) and poly(perfluorodecyl acrylate) nanoparticles with different surface properties in unharmed and brain-traumatized rats. Front Neurol 14:994877 (2023). PubMed: 36814997