Anti-IL-1 beta抗体(ab9722)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IL-1 beta抗体
参阅全部 IL-1 beta 一抗 -
描述
兔多克隆抗体to IL-1 beta -
宿主
Rabbit -
特异性
Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.
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经测试应用
适用于: IHC-P, Sandwich ELISA, WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
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阳性对照
- IHC-P: Human adrenal tissue; Recombinant mouse IL-1 beta protein (ab9723) can be used as a positive control in WB
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
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| WB | (6) |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
靶标
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功能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
组织特异性
Expressed in activated monocytes/macrophages (at protein level). -
序列相似性
Belongs to the IL-1 family. -
翻译后修饰
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
细胞定位
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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数据库链接
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
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别名
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 µg/ml.
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Western blot - Anti-IL-1 beta antibody (ab9722)To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.
Non-reducing conditions.
实验方案
数据表及文件
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Datasheet download
文献 (864)
ab9722 被引用在 864 文献中.
- Hong Y et al. X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity. Genes Dis 11:935-951 (2024). PubMed: 37692514
- Shi B et al. NLRP3 activation in macrophages promotes acute intestinal injury in neonatal necrotizing enterocolitis. World J Pediatr 20:153-164 (2024). PubMed: 37389784
- Lu T et al. Alisol A Exerts Neuroprotective Effects Against HFD-Induced Pathological Brain Aging via the SIRT3-NF-κB/MAPK Pathway. Mol Neurobiol 61:753-771 (2024). PubMed: 37659035
- Yin S et al. RGS5 augments astrocyte activation and facilitates neuroinflammation via TNF signaling. J Neuroinflammation 20:203 (2023). PubMed: 37674228
- Verma N et al. Aβ efflux impairment and inflammation linked to cerebrovascular accumulation of amyloid-forming amylin secreted from pancreas. Commun Biol 6:2 (2023). PubMed: 36596993
