Anti-IL-1 beta抗体(ab2105)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IL-1 beta抗体
参阅全部 IL-1 beta 一抗 -
描述
兔多克隆抗体to IL-1 beta -
宿主
Rabbit -
特异性
In general, this antibody also detects primate IL-1 beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1 alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1 beta precursor in lysates.
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经测试应用
适用于: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFrmore details -
种属反应性
与反应: Human
预测可用于: Non human primates
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免疫原
Recombinant full length protein corresponding to Human IL-1 beta aa 100 to the C-terminus. Produced in E.coli. MW of recombinant IL-1 beta 17kDa, mature chain without propeptide.
Database link: P01584 -
阳性对照
- IHC-P: human medullary lymph node
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常规说明
caution, F(c) domain of rabbit IgG may interact with cells non-specifically.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度
DEAE-Chromatography -
纯化说明
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
|
|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| Functional Studies |
Use at an assay dependent concentration.
|
|
| WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
| IP |
Use at an assay dependent concentration.
1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
|
| Neutralising |
Use at an assay dependent concentration.
Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| IHC-P | (9) |
Use at an assay dependent concentration.
|
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 28726778
|
| 说明 |
|---|
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RIA
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Functional Studies
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. 1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
|
IP
Use at an assay dependent concentration. 1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
|
Neutralising
Use at an assay dependent concentration. Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
|
Flow Cyt
Use at an assay dependent concentration. F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28726778 |
靶标
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功能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
组织特异性
Expressed in activated monocytes/macrophages (at protein level). -
序列相似性
Belongs to the IL-1 family. -
翻译后修饰
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
细胞定位
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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数据库链接
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
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别名
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)Immunohistochemical analysis of Formalin-fixed paraffin-embedded human medullary lymph node sections labeling IL-1 beta with ab2105. Peroxidase goat anti-rabbit at 1/10,000 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)This image is courtesy of an Abreview submitted by Miss Silke Vorwaldab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.
实验方案
数据表及文件
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Datasheet download
文献 (198)
ab2105 被引用在 198 文献中.
- Huang ZX et al. Lipoxin A4 depresses inflammation and promotes autophagy via AhR/mTOR/AKT pathway to suppress endometriosis. Am J Reprod Immunol 89:e13659 (2023). PubMed: 36412044
- Wang G et al. Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1α signaling and glutamine metabolism. Sci Adv 9:eabo7555 (2023). PubMed: 36598999
- Silwal P et al. Chemical mimetics of the N-degron pathway alleviate systemic inflammation by activating mitophagy and immunometabolic remodeling. Exp Mol Med 55:333-346 (2023). PubMed: 36720915
- Owembabazi E et al. Co-administration of alcohol and combination antiretroviral therapy (cART) in male Sprague Dawley rats: A study on testicular morphology, oxidative and cytokines perturbations. Anat Cell Biol 56:236-251 (2023). PubMed: 36759974
- AlQot HE & Rylett RJ A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability. Sci Rep 13:3037 (2023). PubMed: 36810877
