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AB206410

Anti-IL-1 alpha 抗体 [EPR19904-247]

Anti-IL-1 alpha antibody [EPR19904-247]

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(4 Publications)

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

查看别名

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

3 Images
Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green)labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • WB

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

All lanes:

Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

Exposure time: 3s

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • WB

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID : 9785472; PMID : 1868253; PMID : 19104081).

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, <a href='/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa

Observed band size: 15 kDa,20 kDa,34 kDa

false

Exposure time: 15s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19904-247

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).
See full target information IL1A
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 23:3843-3849 PubMed36444597

2022

A Comparison of the Characteristics of Cytokine Storm between Lichen Planus and Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Salmeh Kalbassi,Lida Radfar,Mohammadreza Azimi,Simin Shadanpoor,Ali Ghorbani Ranjbary

NPJ breast cancer 8:30 PubMed35260569

2022

Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew W Chung,Anthony J Kozielski,Wei Qian,Jianying Zhou,Ann C Anselme,Alfred A Chan,Ping-Ying Pan,Delphine J Lee,Jenny C Chang

Frontiers in pharmacology 12:673818 PubMed34093202

2021

An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Jian Shi,Zhaozheng Li,Ling Li,Rui Zhang,Yang Bai,Junmei Li,Fang Liang,Yiting Tang

Cell death & disease 12:402 PubMed33854044

2021

A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Zhaozheng Li,Yang Bai,Rui Zhang,Ran Meng,Fangping Chen,Haichao Wang,Timothy R Billiar,Xianzhong Xiao,Ben Lu,Yiting Tang
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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