Anti-IL-1 alpha抗体(ab9614)
Key features and details
- Rabbit polyclonal to IL-1 alpha
- Suitable for: ICC/IF, Sandwich ELISA, WB, Neutralising, IHC-P, ELISA
- Reacts with: Human
- Isotype: unknown
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IL-1 alpha抗体
参阅全部 IL-1 alpha 一抗 -
描述
兔多克隆抗体to IL-1 alpha -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Sandwich ELISA, WB, Neutralising, IHC-P, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-1 alpha. Corresponds to Human IL-1 alpha, active form without propeptide. Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha).
Database link: P01583 -
阳性对照
- IHC-P: Human liver tissue; Recombinant human IL-1 alpha protein (ab9615) can be used as a positive control in WB.
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常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab7632
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
|
| WB | (1) |
Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. |
|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| ELISA |
Use at an assay dependent concentration.
To detect hIL-1a by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
|
WB
Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ELISA
Use at an assay dependent concentration. To detect hIL-1a by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
靶标
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功能
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
序列相似性
Belongs to the IL-1 family. -
结构域
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
细胞定位
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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数据库链接
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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别名
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (ab9614)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IL-1 alpha with ab9614 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Western blot - Anti-IL-1 alpha antibody (ab9614) -
Sandwich ELISA - Anti-IL-1 alpha antibody (ab9614)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab9614 被引用在 20 文献中.
- Khodadadei F et al. The effect of A1 and A2 reactive astrocyte expression on hydrocephalus shunt failure. Fluids Barriers CNS 19:78 (2022). PubMed: 36171630
- Khan IN et al. Comparison of Interleukin-1 Ligand Expression by Human Papilloma Virus Status in HNSCCs. Head Neck Pathol 16:763-772 (2022). PubMed: 35334093
- Schunk SJ et al. Interleukin-1α Is a Central Regulator of Leukocyte-Endothelial Adhesion in Myocardial Infarction and in Chronic Kidney Disease. Circulation 144:893-908 (2021). PubMed: 34192892
- Tanaka Y et al. Baicalein Inhibits Benzo[a]pyrene-Induced Toxic Response by Downregulating Src Phosphorylation and by Upregulating NRF2-HMOX1 System. Antioxidants (Basel) 9:N/A (2020). PubMed: 32526964
- Guttenplan KA et al. Neurotoxic Reactive Astrocytes Drive Neuronal Death after Retinal Injury. Cell Rep 31:107776 (2020). PubMed: 32579912