Anti-IKAP/p150抗体(ab62498)
Key features and details
- Rabbit polyclonal to IKAP/p150
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-IKAP/p150抗体
参阅全部 IKAP/p150 一抗 -
描述
兔多克隆抗体to IKAP/p150 -
宿主
Rabbit -
特异性
This antibody reacts with IKAP/p150
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经测试应用
适用于: WB, ICC, ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human IKAP/p150.
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阳性对照
- A20 cell lysate and A20 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).
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| ICC |
Use a concentration of 1 - 20 µg/ml.
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| ICC/IF |
Use a concentration of 1 - 20 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa). |
|
ICC
Use a concentration of 1 - 20 µg/ml. |
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ICC/IF
Use a concentration of 1 - 20 µg/ml. |
靶标
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功能
May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. -
疾病相关
Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births. -
序列相似性
Belongs to the ELP1/IKA1 family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 230233 Mouse
- SwissProt: Q7TT37 Mouse
- Unigene: 282743 Mouse
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别名
- DKFZp781H1425 antibody
- DYS antibody
- Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
see all
图片
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Western blot - Anti-IKAP/p150 antibody (ab62498)Lane 1 : Anti-IKAP/p150 antibody (ab62498) at 0.5 µg/ml
Lane 2 : Anti-IKAP/p150 antibody (ab62498) at 1 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG
Predicted band size: 148 kDa
Observed band size: 100,148 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-IKAP/p150 antibody (ab62498)Immunocytochemistry of IKAP/p150 in A20 cells with ab62498 antibody at 1 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-IKAP/p150 antibody (ab62498)Immunofluorescence of IKAP/p150 in A20 cells using ab62498 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62498 被引用在 1 文献中.
- Chen WT et al. Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation. J Biomed Sci 28:81 (2021). PubMed: 34819065