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重组RabMAb

重组Anti-Ihh抗体[EP1192Y] (ab52919)

Reviews (1)Q&A (2)References (32)
Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
  • Anti-Ihh antibody [EP1192Y] (ab52919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1192Y] to Ihh
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-Ihh抗体[EP1192Y]
    参阅全部 Ihh 一抗

  • 描述

    兔单克隆抗体[EP1192Y] to Ihh

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Jurkat, NIH/3T3, C6, His-tagged (IHH) recombinant protein (aa1-411), Human cerebellum lysate. IHC-P: Human liver, Mouse cerebrum, Rat cerebrum.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1192Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52919于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/5000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
说明
WB
1/5000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能

    Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

  • 组织特异性

    Expressed in embryonic lung, and in adult kidney and liver.

  • 疾病相关

    Defects in IHH are the cause of brachydactyly type A1 (BDA1) [MIM:112500]. BDA1 is an autosomal dominant disorder characterized by middle phalanges of all the digits rudimentary or fused with the terminal phalanges. The proximal phalanges of the thumbs and big toes are short.
    Defects in IHH are a cause of acrocapitofemoral dysplasia (ACFD) [MIM:607778]. ACFD is a disorder characterized by short stature of variable severity with postnatal onset. The most constant radiographic abnormalities are observed in the tubular bones of the hands and in the proximal part of the femur. Cone-shaped epiphyses or a similar epiphyseal configuration with premature epimetaphyseal fusion result in shortening of the skeletal components involved. Cone-shaped epiphyses were also present to a variable extent at the shoulders, knees, and ankles.

  • 序列相似性

    Belongs to the hedgehog family.

  • 翻译后修饰

    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.

  • 细胞定位

    Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
  • Information by UniProt

  • 数据库链接

    • 别名

      • BDA1 antibody
      • HHG-2 antibody
      • HHG2 antibody
      • IHH antibody
      • IHH_HUMAN antibody
      • Indian Hedgehog antibody
      • Indian hedgehog homolog antibody
      • Indian hedgehog protein antibody
      • Indian hedgehog protein C-product antibody
      • Indian hedgehog protein N-product antibody
      see all

    图片

    • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      All lanes : Anti-Ihh antibody [EP1192Y] (ab52919) at 1/5000 dilution

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
      Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lane 1, Lane 3: 10 seconds, Lane 2: 80 seconds.

      Primary and secondary antibody: incubated for 1h at room temperature.

    • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution + Human cerebellum lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG. at 1/2000 dilution

      Predicted band size: 45 kDa


      Exposure time: 10 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Primary and secondary antibody: incubated for 1h at room temperature.

    • Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Western blot - Anti-Ihh antibody [EP1192Y] (ab52919)
      Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution + His-tagged human Indian Hedgehog (IHH) recombinant protein (aa1-411) at 10 ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Primary and secondary antibody: incubated for 1h at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)

      The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/200 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Human liver.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)

      The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Mouse cerebrum.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (ab52919)

      The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Rat cerebrum.

    • Anti-Ihh antibody [EP1192Y] (ab52919)
      Anti-Ihh antibody [EP1192Y] (ab52919)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (32)

    发表研究结果有使用 ab52919？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab52919 被引用在 32 文献中.

    • Hagizawa H  et al. Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone. Front Cell Dev Biol 11:1151947 (2023). PubMed: 37255604
    • Deng H  et al. Altered Expression of the Hedgehog Pathway Proteins BMP2, BMP4, SHH, and IHH Involved in Knee Cartilage Damage of Patients With Osteoarthritis and Kashin-Beck Disease. Cartilage 13:19476035221087706 (2022). PubMed: 35313742
    • Tsukasaki M  et al. Periosteal stem cells control growth plate stem cells during postnatal skeletal growth. Nat Commun 13:4166 (2022). PubMed: 35851381
    • Zhou X  et al. CRC Therapy Identifies Indian Hedgehog Signaling in Mouse Endometrial Epithelial Cells and Inhibition of Ihh-KLF9 as a Novel Strategy for Treating IUA. Cells 11:N/A (2022). PubMed: 36552817
    • Bao J  et al. Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2a/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells. Front Cell Dev Biol 9:675486 (2021). PubMed: 34164397
    View all Publications for this product

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Ihh antibody [EP1192Y]

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Chondrocytes)
    Specification
    Chondrocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Jan 27 2011

    Question

    Phone call requesting information on if the Anti-Ihh antibody [EP1192Y] (ab52919) is likely to cross react with the protein derived from bovine samples.

    Read More
    Answer

    I have tried to check the likelihood of the Anti-Ihh antibody [EP1192Y] (ab52919) being able to cross-react with the protein from bovine samples. Unfortunately, the only protein sequence I have been able to find for the bovine Ihh protein is of the Uniprot reference http://www.uniprot.org/uniprot/Q08D77. This seems to be N-truncated, or be naturally shorter than the human and mouse protein. As the antibody ab52919 has been raised against a peptide derived from the N-terminal region, I cannot say whether it would be able to cross-react or not. From the sequence I have access to it seems it may not.

    However, we do have an alternative antibody which may work better for you. This is the Rabbit polyclonal to Ihh, ab39634. This has been raised against a peptide taken from the C-terminus of the mouse protein, which shows high homology with the bovine sequence (please refer to the sequence alignment attached) and I would therefore expect it to cross react. However, as we have not tested this ourselves I would not be able to guarantee this. If you would be interested, you would qualify for our Abtrial scheme. More information on this offer can be found here:

    www.abcam.com/abtrial

    Read More

    Question

    Vielen dank,
    nun habe ich Ihre mail erhalten und die Fragen beantwortet,ich sende sie
    anbei zurück und bitte Sie herzlich um rasche Bearbeitung und Antwort.
    Vielen dank
    Herzliche Grüße

    Read More
    Answer

    Ich habe Ihre Ergebnisse an das Labor, das diesen Antikoerper herstellt, weitergeleitet.
    Es wird nun nachgeforscht, wo das Problem liegt.
    Da ich nicht moechte,dass Sie so lange warten muessen, wuerde ich Ihnen gernen einen alternativen Antikoerper als Ersatz anbieten. Oder auch eine Gutschrift, wenn Sie das bevorzugen.
    Es tut mir sehr leid, dass dieser Antikoeprer nicht den Beschreibungen auf dem Datenblatt entsprochen hat. Ich moechte mich auch dafuer bedanken, dass Sie uns auf dieses Problem aufmerksam gemacht haben.
    Bitte lassen Sie mich wissen, welchen Antikoerper Sie gerne als Ersatz haetten.
    Ich bin gerade in unserer amerikanischen Niederlassung: Bitte entschuldigen Sie die fehlenden Umlaute.
    Ich freue mich auf Ihre Antwort und verbleibe

    Read More

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