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AB52919

Anti-Ihh 抗体 [EP1192Y]

Anti-Ihh antibody [EP1192Y]

2

(1 Review)

|

(39 Publications)

Rabbit Recombinant Monoclonal IHH antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 39 publications.

查看别名

Indian hedgehog protein, IHH, HHG-2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/200 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Human liver.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Rat cerebrum.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ihh antibody [EP1192Y] (AB52919)

The paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab52919 at 1/1000 dilutions for 30 mins at room temperature and followed Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND© RX instrument. Counterstained with hematoxylin. Positive staining on the Mouse cerebrum.

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)
  • WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibody : incubated for 1h at room temperature.

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution

All lanes:

Human cerebellum lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 45 kDa

false

Exposure time: 10s

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)
  • WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1, Lane 3 : 10 seconds, Lane 2 : 80 seconds. Primary and secondary antibody : incubated for 1h at room temperature.

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (ab52919) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)
  • WB

Supplier Data

Western blot - Anti-Ihh antibody [EP1192Y] (AB52919)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibody : incubated for 1h at room temperature.

All lanes:

Western blot - Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution

All lanes:

His-tagged human Indian Hedgehog (IHH) recombinant protein (aa1-411) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

Exposure time: 180s

不同偶联物与剂型 (8)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1192Y

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Indian hedgehog (Ihh) protein known by its gene symbol IHH plays key roles in cell signaling. Ihh with an approximate mass of 45 kDa belongs to the Hedgehog family of signaling molecules. It is expressed in various tissues including cartilage bone and parts of the gastrointestinal tract where it regulates growth and patterning. Ihh acts by binding to its receptor Patched triggering downstream signaling that influences cell fate decisions.
Biological function summary

Ihh is significant in regulating the differentiation and proliferation of chondrocytes in the growth plate of long bones. It acts as part of a complex regulatory network coordinating with Parathyroid hormone-related protein (PTHrP) to maintain proper development of endochondral bones. Ihh also influences epithelial and mesenchymal interactions during embryo development evidencing its important role in developmental biology.

Pathways

Ihh is a critical component of the Hedgehog signaling pathway a conserved pathway involved in embryonic development and adult tissue homeostasis. This pathway also includes proteins like Sonic hedgehog (Shh) and Desert hedgehog (Dhh) as well as the receptor Smoothened which mediates the signaling cascade. Ihh signaling impacts pathways such as the Wnt pathway influencing the proliferation and differentiation of cells thereby highlighting its integration into broader cellular signaling networks.

Ihh misregulation can lead to skeletal disorders like brachydactyly type A1 characterized by shortened fingers and toes. Furthermore abnormal Ihh signaling can contribute to certain cancers such as chondrosarcoma where overactive Hedgehog signaling promotes tumorigenesis. The relationship between Ihh and other Hedgehog proteins including Shh is key in understanding these pathologies as they share pathways that are often disrupted in disease scenarios.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Plays a role in embryonic morphogenesis; it is involved in the regulation of endochondral skeleton formation, and the development of retinal pigment epithelium (RPE), photoreceptors and periocular tissues (By similarity).. Indian hedgehog protein. The C-terminal part of the indian hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). Both activities occur in the endoplasmic reticulum (By similarity). Plays a role in hedgehog paracrine signaling (PubMed : 24342078). Associated with the very-low-density lipoprotein (VLDL) particles to function as a circulating morphogen for endothelial cell integrity maintenance (PubMed : 20839884).. Indian hedgehog protein N-product. The dually lipidated indian hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity). Plays a role in morphogenesis of the skeleton by coordinating growth and differentiation of the endochondral skeleton (By similarity). Positively regulates PTHLH expression during endochondral bone formation preventing chondrocyte hypertrophy. In contrast, participates in normal chondrocyte proliferation in a PTHLH-independent pathway (By similarity).
See full target information IHH
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文献 (39)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Nature communications 16:4344 PubMed40346121

2025

Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Li,Zhiyuan Shi,Huaqing Jing,Yunsheng Dou,Xinyi Liu,Mengyao Zhang,Zitong Qiu,Zbynek Heger,Nan Li

Cellular and molecular life sciences : CMLS 81:74 PubMed38308696

2024

Intervertebral disc-intrinsic Hedgehog signaling maintains disc cell phenotypes and prevents disc degeneration through both cell autonomous and non-autonomous mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Siyuan Hu,Chunmei Xiu,Meng Li,Yixin Zheng,Rui Zhang,Bin Li,Jianquan Chen

Frontiers in cell and developmental biology 11:1151947 PubMed37255604

2023

Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Hagizawa,Saeko Koyamatsu,Seiji Okada,Takashi Kaito,Noriyuki Tsumaki

Biology of reproduction 108:241-257 PubMed36525341

2022

Hedgehog signaling regulates Wolffian duct development through the primary cilium†.

Applications

Unspecified application

Species

Unspecified reactive species

Maíra Bianchi Rodrigues Alves,Laura Girardet,Céline Augière,Kyeong Hye Moon,Camille Lavoie-Ouellet,Agathe Bernet,Denis Soulet,Ezequiel Calvo,Maria E Teves,Charles Joly Beauparlant,Arnaud Droit,Alexandre Bastien,Claude Robert,Jinwoong Bok,Barry T Hinton,Clémence Belleannée

Cells 11: PubMed36552817

2022

CRC Therapy Identifies Indian Hedgehog Signaling in Mouse Endometrial Epithelial Cells and Inhibition of Ihh-KLF9 as a Novel Strategy for Treating IUA.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhao Zhou,Yiyi Kang,Yuntzu Chang,Siyu Xia,Ming Wu,Jun Liu,Dirong Dong,Wei Zhang,Hong Chen,Hui Li

Nature communications 13:4166 PubMed35851381

2022

Periosteal stem cells control growth plate stem cells during postnatal skeletal growth.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Tsukasaki,Noriko Komatsu,Takako Negishi-Koga,Nam Cong-Nhat Huynh,Ryunosuke Muro,Yutaro Ando,Yuka Seki,Asuka Terashima,Warunee Pluemsakunthai,Takeshi Nitta,Takashi Nakamura,Tomoki Nakashima,Shinsuke Ohba,Haruhiko Akiyama,Kazuo Okamoto,Roland Baron,Hiroshi Takayanagi

Cartilage 13:19476035221087706 PubMed35313742

2022

Altered Expression of the Hedgehog Pathway Proteins BMP2, BMP4, SHH, and IHH Involved in Knee Cartilage Damage of Patients With Osteoarthritis and Kashin-Beck Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Deng,Xiang Xiao,Mumba Mulutula Chilufya,Lichun Qiao,Yizhen Lv,Ziwei Guo,Jian Lei,Jiaxin Liu,Yan Zhao,Jiaheng Zhang,Wenyue Wang,Jing Han,Chengjuan Qu

Frontiers in cell and developmental biology 9:675486 PubMed34164397

2021

Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junping Bao,Zhanyang Qian,Lei Liu,Xin Hong,Hui Che,Xiaotao Wu

Clinical and experimental dermatology 46:1052-1057 PubMed33714217

2021

Expression of Hedgehog signalling molecules in microcystic adnexal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

T Gambichler,I Hartenstein,M Dreißigacker,E Stockfleth,M Stücker,J Schaller,H-J Schulze,J C Becker,H U Käfferlein,T Brüning,K Lang
View all publications
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