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重组RabMAb

重组Anti-IGJ抗体[SP105] (ab105229)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
  • Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
  • Anti-IGJ antibody [SP105] (ab105229)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP105] to IGJ
  • Suitable for: IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-IGJ抗体[SP105]
    参阅全部 IGJ 一抗

  • 描述

    兔单克隆抗体[SP105] to IGJ

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human IGJ aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P01591

  • 阳性对照

    • IHC-P: Human tonsil and stomach tissue Flow Cyt (Intra): Daudi cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Protein A/G purified

  • 纯化说明

    Purified from TCS by protein A/G.

  • 克隆

    单克隆

  • 克隆编号

    SP105

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab105229于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt (Intra)
1/20.
说明
IHC-P
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt (Intra)
1/20.

靶标

  • 功能

    Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • IGCJ antibody
      • IGJ_HUMAN antibody
      • Immunoglobulin J chain antibody
      • Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody
      • immunoglobulin joining chain antibody
      • JCH antibody
      • JCHAIN antibody
      • Joining chain of multimeric IgA and IgM antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab105229 for 10 mins at room temperature.

    • Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229)
      Flow Cytometry (Intracellular) - Anti-IGJ antibody [SP105] (ab105229)

      Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab105229 for 10 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
      ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Anti-IGJ antibody [SP105] (ab105229)
      Anti-IGJ antibody [SP105] (ab105229)

    文献 (1)

    发表研究结果有使用 ab105229？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab105229 被引用在 1 文献中.

    • Wang M  et al. IGJ suppresses breast cancer growth and metastasis by inhibiting EMT via the NF‑κB signaling pathway. Int J Oncol 63:N/A (2023). PubMed: 37539706

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGJ antibody [SP105]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica ER2
    Permeabilization
    No
    Specification
    small intestine
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Masahiro Hirata

    Verified customer

    提交于 May 10 2021

    Question

    No staining on human colon and appendix sections.

    Read More
    Answer

    Thank you for your call yesterday and for letting us know about the trouble with ab105229.

    I've heard back from the lab, and we have not received other reports of the antibody not working, so it's possible that the protocol can be altered to produce better results. The only alterations that we would suggestare to increasethe antigen retrieval and primary antibody incubation times. We also have not tried the antibody with colon and appendix sections, so if you have any tonsil slides we would suggest using them as a positive control. You may also want to try an antibody that you've previously optimized on the colon or appendix tissue, just to make sure the tissue is not too old.

    As we discussed, if the staining results do not improve, I would be happy to send a different IGJ antibody free of charge.

    Please keep me updated about any progress, and let me know if you have any questions or need anything else from me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com