重组Anti-IgG抗体[R10Z8E9] (ab124055)
![Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.cn/ps/products/124/ab124055/Images/ab124055-412863-AntiIgG-antibody-R10Z8E9-western-blot-serum-human.jpg "Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [R10Z8E9] to IgG
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IgG抗体[R10Z8E9]
参阅全部 IgG 一抗 -
描述
小鼠单克隆抗体[R10Z8E9] to IgG -
宿主
Mouse -
经测试应用
适用于: Indirect ELISA, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Indirect ELISA: Human IgG1. WB: Human serum lysate.
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常规说明
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
R10Z8E9 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124055于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
|
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| WB |
1/1000. Predicted molecular weight: 36 kDa.
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| 说明 |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
|
WB
1/1000. Predicted molecular weight: 36 kDa. |
靶标
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细胞定位
Secreted -
数据库链接
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
- SwissProt: P01860 Human
- SwissProt: P01861 Human
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别名
- Ig gamma 1 chain C region antibody
- Ig gamma 2 chain C region antibody
- Ig gamma 3 chain C region antibody
see all
图片
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Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366)
Predicted band size: 36 kDa
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Expected MW: 50 kDa.
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![Indirect ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.cn/ps/products/124/ab124055/Images/ab124055-412862-AntiIgG-antibody-R10Z8E9-elisa-human1.jpg)
Indirect ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
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![Anti-IgG antibody [R10Z8E9] (ab124055)](https://www.abcam.cn/ps/products/124/ab124055/Images/ab124055-5-benefits-of-recombinant-antibodies.png)
Anti-IgG antibody [R10Z8E9] (ab124055)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab124055 被引用在 7 文献中.
- Wang H et al. AADAC promotes therapeutic activity of cisplatin and imatinib against ovarian cancer cells. Histol Histopathol 37:899-907 (2022). PubMed: 35451495
- Mukherjee S et al. Cross-talk between mutant p53 and p62/SQSTM1 augments cancer cell migration by promoting the degradation of cell adhesion proteins. Proc Natl Acad Sci U S A 119:e2119644119 (2022). PubMed: 35439056
- Contreras CJ et al. RIPK1 and RIPK3 regulate TNFα-induced β-cell death in concert with caspase activity. Mol Metab 65:101582 (2022). PubMed: 36030035
- Chi Y et al. Exosomal lncRNA UCA1 Derived From Pancreatic Stellate Cells Promotes Gemcitabine Resistance in Pancreatic Cancer via the SOCS3/EZH2 Axis. Front Oncol 11:671082 (2021). PubMed: 34868904
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Zhang J et al. RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons. J Biol Chem 293:19078-19091 (2018). PubMed: 30327427
- Laumet G et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat Neurosci 18:1746-55 (2015). PubMed: 26551542