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Anti-IGFBP4抗体(ab77350)

Reviews (2)Q&A (1)References (3)
Western blot - Anti-IGFBP4 antibody (ab77350)

    Key features and details

    • Goat polyclonal to IGFBP4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-IGFBP4 antibody [EPR19762] (ab205581)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-IGFBP4抗体
      参阅全部 IGFBP4 一抗

    • 描述

      山羊多克隆抗体to IGFBP4

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Rat, Sheep, Goat, Chicken, Cow, Pig

    • 免疫原

      Synthetic peptide:

      KGELDCHQLADSFRE

      , corresponding to C terminal amino acids 244-258 of Human IGFBP4 (NP_001543.2).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab77350于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    说明
    应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/8000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    图片

    文献 (3)

    发表研究结果有使用 ab77350？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab77350 被引用在 3 文献中.

    • Madani AY  et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
    • Contois LW  et al. Insulin-like growth factor binding protein-4 differentially inhibits growth factor-induced angiogenesis. J Biol Chem 287:1779-89 (2012). WB ; Human . PubMed: 22134921
    • Gardner S  et al. TGF-ß inhibits muscle differentiation by blocking autocrine signaling pathways initiated by IGF-II. Mol Endocrinol 25:128-37 (2011). WB ; Mouse . PubMed: 21106882

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IGFBP4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (murine breast cancer cell line monolayer)
    Permeabilization
    Yes - 0,01 % TritonX-100
    Specification
    murine breast cancer cell line monolayer
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 08 2019

    Western blot abreview for Anti-IGFBP4 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (serum- 3 microliter)
    Specification
    serum- 3 microliter
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ru Contois

    Verified customer

    提交于 Nov 11 2009

    Question

    I would like to test ab77350 in IHC-P.

    Read More
    Answer


    I am very pleased to hear you would like to accept our offer and test ab77350 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com